Quantitative Expression Of Apoptosis Inhibitor Survivin MRNA In Nasopharyngeal Carcinoma

Objective To study the expression of survivin mRNA and the relationship between survivin mRNA and clinical pathological features in Nasopharyngeal carcinoma (NPC).Methods The expression of survivin mRNA in 46 samples of nasopharyngeal carcinoma tissues and 18 samples of normal nasopharyngeal tissues were detected by FQ-PCR,using glyceraldehydes-3-phophate dehydrogenase (GAPDH) as internal standard. On the principle that the CT value is in inverse proportion to the log value of original copy number of target sequence, SURVIVIN CT/ GAPDH CT was used to evaluate the mRNA expression level in different tissues. Results The survivin mRNA levels in nasopharyngeal carcinoma tissues (1.037±0.133) was significantly higher than those in normal nasopharyngeal tissues (1.187±0.092), (P=0.000).By statistical analysis , there was no relationship between survivin mRNA and sex, age, TNM stages, lymph node metastases(P>0.05). Conclusions The expression of survivin mRNA is higher in nasopharyngeal carcinoma tissues than in normal nasopharyngeal tissues. The expression of survivin mRNA in nasopharyngeal carcinoma may play an important role in tumorigenesis through inhibition of apoptosis. FQ-PCR has the characteristics of the real-time detection,good repetition,high sensitivity and high specificity through detecting survivin in NPC.