| Objective To observe the differentiation of neural stem cells(NSCs) from fetal human brain with retinoic acid pretreatment in the spinal cord injured rats and the recovery of hindlimb motor function. Methods Proliferated populations of undifferentiated NSCs isolated from the embryonic week 10 human cerebral cortex. Parts of the cells were exposed to retinoic acid for 6 days before transplantation. Thirty adult SD rats were used and Impact injury was induced 6 days before transplantation, hind limb motor function was assessed once a week. Experimental group was randomly divided into two groups: (l)one group transplanted the NSCs with retinoic acid pretreatment into the rat spinal cord;(2) the other transplanted the NSCs without retinoic acid pretreatment. Control group was injected with the same dose of PBS. Histological analysis 4 weeks later showed the differentiation of the NSCs. Result The NSCs survived well in the injured spinal cord ,and can migrate away from the injection site, part of them differentiated into neural cells. The experimental group got higher scores than control group. The cells exposed to retinoic acid can express neuronal maker-NF, transplantation of these cells gained an advantage of promotion functional recovery. Conclusions The cells with retinoic acid pretreatment candifferentiate into proper neural cells in vivo and can promote functional recovery after transplantation .Therefore, as an ideal source of transplantation, they may play a great role in clinical treatment for patients with spinal cord injury. |
PDF Full Text Request