Study On Expression And Correlation Of EIF4E, VEGF And BFGF In Laryngeal Squamous Cell Carcinoma

Objective: The expression and correlation of eIF4E,VEGF and bFGF were studied by detection their expression in laryngeal squamous cell carcinoma (LSCC).Methods: An immunohistochemical analysis with antibodies to eLF4E, VEGF and bFGF was performed on 52 specimens of the LSCC, 20 specimens of dysplasia, 30 specimens of hyperplasia and 10 specimens of normal mucosa; Western-blot analysis was performed on tumor core, transition core, "tumor-free" core of 20 specimens of fresh LSCC using anti-eIF4E, VEGF,bFGF antiserums.Results: All 52 patients (100%) overexpressed eIF4E in the LSCC, 39 of 52 patients (75.0%) were VEGF positive and 44 of 52 patients (82.7%) had bFGF overexpression, they were all remarkably higher than that in the control groups. The overexpressions of VEGF and bFGF were significantly associated with node metastasis. The expression of eIF4E and bFGF protein level from "tumor-free" core, transition core and tumor core of LSCC appeared progressive overexpression; It appered that there was significant elevation of the VEGF protein level in tumor core compared with tumor transition and "tumor-free" core; there appered to be statistically significantly correlation of eIF4E, VEGF and bFGF expression.Conclusion: Overexpression of elF4E may be a molecular marker of malignant transformation and progression; The overexpression of VEGF was highly associated with the growth and metastasis of LSCC, and may be of prognostic value; The over- expression of bFGF played a key role on growth and metastasis of LSCC, and showed stastically significally correlation with malignacy progression; The correlation of eIF4E, VEGF and bFGF may serve as a potential target for antiangiogenic therapy of LSCC.