The Study Of Moxibustion Separated By Herbal Cake On Experimental Endometriosis In The Rat

Objective To establish the experimental endometriosis in rats, to study the ectopic endometrum's histological structure change and to explore the mechanism of the effect of moxibustion separated by herbal cake on the treatment of endometriosis.Methods 60 SD rats were randomly divided into two groups: the normal and the experimental. 51 SD rats were induced by surgery for the experimental endometriosis. After the model be made, rats in the experimental group were divided into endomrtriosis model group(EMM), moxibustion separated by herbal cake group(MSBHC), mild moxibustion group (MM), and Danazol group to treat for thirty days respectively. Then, the levels of Interleukin-6(IL-6) in the blood serum and prostaglandinE2 (PGE2) in the blood plasma of all groups were measured by radioimmunoassay(RIA); the ectopic endometrium's focuses were observed by microscope and eyes.Results (1)Compared with the normal group, the levels of IL-6 in the blood serum and PGE2 in the blood plasma of EMM group were significantly higher (P<0.01 and P<0.005 respectively). (2)After treatment, the levels of IL-6 in the blood serum in the three treated groups were degraded significantly as compared with those of EMM group (P<0.01, P<0.01 and P<0.05 respectively), but without statistical meaning for the changes of IL-6 levels between these groups (P>0.05); (3)The levels of PGE2 in the blood plasma of three treated groups became obviously lower than those of EMM group, which showed very significance (P<0.005, P<0.005 and P<0.01 respectively); there was no difference between MSBHC group and Danazol group (P>0.05), but these two groups had differencefrom MM group(P<0.05, P<0.05 respectively ). (4)As eyes observing, the volume of ectopic endometrium in rats of three treated groups shrinked comparing with those of the EMM geoup; and there were significance between the MSBHC group and the MM group( P<0.01). The ectopic endometrium's cysts or nodes shrinked or dissolved, the surface blood vessels reduced, so these showed that the three methods all could significantly improve the ectopic endometrium's focuses (P<0.05) in observation for appearance, especially markedly in the MSBHC group and the Danazol group(.P<0.01, P<0.05 respectively ), but no difference between the two groups. (5)There were less counts shrinked glands, small glandular cavity and stromal cells and obsoleted blood dot by microscope observation in three treated groups.Conclusion It is suggested that the therapy of moxibustion separated by herbal cake similar Danazol might regulate the immune disturbance in endometriosis and inhibit the ectopic endomrtrium's hyperplastic and develop, promoted their shrink atrophy by decreasing the levels of IL-6 in the blood serum and PGE2 in the blood plasma to treat endometriosis; so it's one of effective methods in clinical practice for treantment on endometriosis and superior to the mild moxibustion method.