An Experimental Research Of Aspirin On H₂O₂-induced Lenticular Opacity Of Rabbits

Objective: To evaluate the effect of Aspirin on protecting lens from oxidative stress. Methods: 1.Cultured rabbit lenses were treated with 250μM H2O2 to establish cataract models in vitro, then the morphological change of epithelial cells were observed by transmission electron microscope. 2.Rabbit lenses cultured in vitro were divided into 5 group: control group, oxidative stress group, 10mM,20mM and 30mM Aspirin treatment groups. The transparency of all lenses was examined after above lenses incubated for different time.3.After incubation for 24 hr , the extractioner of lens epithelial cell nuclei was analysed by agarose gel electrophoresis; the percent DNA fragmentation of lens epithelial cell was determined by diphenylamine (DPA) method.Result: 1.Under the transmission electron microscope it could be observed that cellular membrane was intact, structure of cellular organs was unclear, and chromatin gathered in the peripheral nucleus of rabbit lens epithelial cell. 2.Aspirin could apparently inhibit H2O2-induced lenticular opacity of rabbit cultured in vitro. 3.The result of agarose gel electrophoresis showed that the pattern of DNA ladder appeared in the extractioner of lens epithelial cell nuclei in oxidative stress group and Aspirin treatment groups. 4.The lever of percent DNA fragmentation was decreased apparently in 10mM,20mM and 30mM Aspirin treatment groups compared with that in oxidative stress group( P < 0.05 ).Conclusion: Aspirin plays an inhibitory role on H2O2-induced rabbit lens epithelial cell apoptosis and can delay and ameliorate the occurrence and development of lenticular opacity.