The Animal Model Development Of Syndrome Of Earth-drying And Water-exhaustion In Rabbits

Purpose:To develop the animal model of Syndrome of Earth-drying and Water-exhaustion in rabbits by multifactor interventional methods.Methods:The experimental rabbits were divided into three groups randomly (Control group, Model group and Model-proving group).Normal feed was given to the animals of Control group, and the Bismuth Subcarbonate feed was given in Model groups for 2 days, no water in the second day; then Furosemide was injected venously twice for dehydration.The animals of the Model group were divided into 2 groups randomly(Endotoxin for 1-time Group and Endotoxin for 2-times Group). low, middle and high dosage of Endotoxin was injected venously respectively in Endotoxin for 1-time Group,then in Endotoxin for 2-times Group ,this manipulation was repeated after 24 hours with the same dosage .Group A and group B were divided northerly in each small group,of which group A was the model-experimenting group,group B was the model-treating group,before and after the injection of Endotoxin , the Normal Saline and Dachengqi Decoction were given orally in A and B groups separately .In the Model-proving groups ,the Dachengqi Decoction was given immediately after the Bismuth Subcarbonate , Furosemide and Endotoxin were administered,while the Normal Saline was given in the Control group for demands.Results: In the Group 3 , Group5 and Group 6,there all came the dead animals ,and the results of other groups were summarized as follows:There were obvious changes of temperature and abdominal circumference in the Model group A, of which Group 2A was the most serious one. The WBC counts and the contents of NO, MDA and MMS increased in various degrees in Group A ,whereas the contents of SOD and -SH decreased ; The contents of K and Na decreased in various degrees in Model groups ; In Model group A, the content of t-PA decreased while the content of PAI-1 increased to some extent, simultaneously the contents of TXB2, 6-keto-PGF1 and T/K value increased in various degrees. There were no or slight differences in most of Model-provinggroups when compared with the Control groups, while the significant difference were found between group A and group B(p<0.05 or p<0.01 ),especially the difference between group 2A and group 2B(p<0.01 ), and significant differences(p<0.05 or p<0.01 ) were found when compared group 2A with group 1A and group 4A respectively.Pathological observatiomof the Model groups,most animals had macular bleeding spots under the skin ,there were obvious tumefaction, congestion and hemorrhage in most of organs and tissues to some extent. The dead animals in this experiment had the most serious pathological changes. Microscopic observation:pathological changes were found in the lungs, livers and intestines with various degrees in the Model groups ,such as cellular degeneration and necrosis ,congestion and hemorrhage of the tissues,infiltration of inflammatory cells .The pathological changes of Group 2A was the most obvious and serious one, slight changes were found in the Model B groups and Model-proving groups, whereas there were no obvious pathological changes in the Control groups.Conclusion: 1 . Undergoing the multifactor intervention of the Bismuth Subcarbonate, Furosemide and middle dosage of Endotoxin for 1-time given, the course of the onset , the main sumptoms and signs and pathological changes of this Model group have the striking interrelationship with the "Syndrome of Earth-drying and Water-exhaustion" of the theories in TCM ,so this model-developing method can be believed successrul.2, The Dachengqi Decoction can not only decrease the temperature and the abdominal circumference, alleviate the symptoms and signs of the rabbits effectively, but also can improve various indexes and pathological changes which are accord with the pathological characteristics in TCM. Those results of the treatment have proved the existence of the model of "Syndrome of Earth-drying and Water-exhaustion", and also indicate the reliability of this model-developing method.