| Objective: Having the control of conventional Modified Kessler suture technique, this experiment was designed to explore the healing process and adhesion degree of the tendons which had been sticked with OB glue, to evaluate the feasibility of repairing the injured tendons with OB glue and preventing adhesion, to supply experimental foundation for clinic finding a new, simple and speedy method of tendon repair.Methods: 1. 42 laiheng hens , which were normal and healthy, were used in the experiment. According to different repairing methods, they were divided into three groups: Group A, using the method of Modified Kessler suture technique after the tendon had been severed; Group B, after the tendon was sutured, using OB glue to coat the sutured tendon and the length was 0.5 cm; Group C, without being sutured, used the method that was to smear with OB glue after the ends had been joined. 2. 1,2,3,4,6,8 weeks after the operation, three hens were killed in every group. Theconditions of tendon healing and adhesion were observed. 3. Every section was used for HE staining, Masson' staining. The conditions of tendon were observed under light microscope, the function of cells was observed under transmission electron microscope and scanning electron microscope. 4. The picture analysis of the sections was dealt with ZL cell parameter analysis system. Put the data into computer and analysis. 5. 2,4,6,8 weeks after the operation, five hens were killed in every group. The tendon' s breaking strength in different period was observed and analysis.Results: 1. There was adhesion of different degree in every group. According to the degree of adhesion, the adhesion was distinguished into â… ,â…¡,â…¢,â…£,â…¤ five grade. In the same period after operation , there was a tendency among A,B,C groups: the adhesion degree of group B and group C was slighter than that of group A, much of the adhesion was â… ,â…¡ grade. However, the adhesion of group A was â…£,â…¤grade. Upon the condition of tendon healing ,the speed of group C was faster than that of group A and group B. 2. The healing process was similar in every group under light microscope, but the healing quality and speed was different. Compared to group A in the same period, the proliferation of fibroblast in group B and group C was more active, the quantity of fibroblast which had changed into tendon cell was larger, the amount of collagen fiber was more, the arrangement of collagen fiber was more regular. 3.Under the transmission electron microscope and scanning electron microscope, in the same period, the cell organelles in the fibroblast of group B and group C was richer; the amount of collagen fibrils was more; the arrangement of collagen fibrils was more regular than group A . But in the fibroblast of group A, the cell organelles wan much less and many cell organelles had the transformations which were dilation, deformation and so on, thus it made the collagen synthesis less and the arrangement of collagen fiber more irregular. 4. The picture analysis showed that, on the total area of fibroblast and the amount of collagen fiber, there was statistically significant difference between group A and group B, C in the same period. However, there was not statistically significant difference between group B and group C. 5. Tensile strength tests revealed that: there were no detectable differences in biomechanical parameters between group A and group B. In group C, two weeks after operation, the tensile strength was significantly less than that of group A and B. Strength increased with time, after four weeks, there was not statistically significant difference among the groups.Conclusion: 1.In the tendon healing process, the fibroblast was young and the proliferation was active, the ability of collagen synthesis was strong in the early period. Then the fibroblast was mature gradually and changed into tendon cell, its quantity decreased gradually, the ability ofcollagen synthesis was weaken; Collagen fiber changed from fine to thick, it was confusion in the... |
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