A Study Of The Relationship Between The Expression Of Connexin43,E-cadherin And Biological Behaviors Of Human Laryngeal Cancer

[Object] To investigate expression of connexin43(Cx43) andE-cadherin (E-cad) in Laryngeal carcinomas and its relationship with the biological behavior.[Method] Immunohistochemistry staining (Picture?two steps method)was used to detect connexin43 and E-cadherin expression in 60 paraffin-embedded laryngeal carcinomas and 10 normal epithelia around tumors. All datas were calculated by statistics.[Result] Cx43 and E-cad were expressed by normal epithelia withtypical membranous staining. 10 normal epithelia strongly expressed Cx43 and E-cad. The reduction rate of Cx43 expression in laryngeal carcinomas was 30%. The significant relationship was observed between low Cx43 expression and dedifferentiation, lymph node metastasis and recurrence (X2 =4.04,6.41,3.86;P<0.05,0.05,0.05), but there was no significance between low Cx43 expression and prognosis (X2=2.65;P> 0.05) .41.67% Laryngeal carcinoma showed reduced or no expression of E-cad. Tumor cell dedifferentiation correlated with reduced expression for E-cad(X2=4.31,p<0. 05). Absent or low E-cad expression was observed more frequently in patients with local recurrence and lymph node metastasis and with less than 5-year survival period(X2 =5.35,6.65,5.14;p<0. 05, 0.01,0. 05).[Conclusion] laryngeal cancer presented inactivation of Cx43 geneand E-cad gene and down regulation of Cx43 and E-cad proteins. The level of Cx43 and E-cad may be a sensitive predictor ofdedifferentiation, invasion, lymph node metastasis and recurrence. E-cad is also a prognostic factor for laryngeal cancer survival.Postgraduate :Sun wenhai Directed by Ji xiangrui,Li yujun...