| Egg oil of Rana temporaria chensinensis David (EORTCD) is the liposoluble part extracted from Rana temporaria chensinensis David by supercritical fluid extraction of CO2 and short path distillation. The components of EORTCD was determined by chromatography methods. The results manifested that there are fatty acid part and fatty amides part in EORTCD. Hexadecanamide, oleamide and some other fatty amide have been identified.In order to search the pharmacological function of EORTCD, the spontaneous activity of mice were monitored, the cooperation of EORTCD with pentobarbital sodium or ethanol was observed, convulsion model induced by strychnine was also set up and the anti-anxiety of EORTCD in the elevated plus-mazetest was introduced in the experiment. The blood lipid, blood pressure, electrocardiogram and respiration were measured after EORTCD administration. The injure of 50Co radiation was used to evaluate the anti-radiation function of EORTCD, tumor growth, brain MDA, GSH assay, immune inhibition models were accepted in the test. The results showed that EORTCD exerted a strong inhibition to CNS, and the anti-convulsion action of EORTCD was observed. EORTCD also reduced blood lipid, inhibited the growth of tumor, and against injure of radiation and cleaning free radical.EORTCD showed a potent inhibition in convulsion induced by strychnine, pentylenetetrozole and picrotoxin. The pharmacodynamics andefficacy of EORTCD was tested by comparing with Diazepam, Alprazolam and EORTCD. It was showed that EORTCD was more effective than Diazepam and Alprazolam in the prolongation of latency in convulsing mice, and it got the same with Diazepam and Alprazolam in the comparation in efficacy. Diazepam and Alprazolam showed toxicity in higher dose in mice. Though EORTCD is used in higher dose than Diazepam or Alprazolam, it is effective and safer.The strychnine-induced convulsion model was also introduced to study the active components of EORTCD. The result was that fatty amides among EORTCD showed potent anticonvulsion. Palmitamide exhibited a potent molecule in anticonvulsion test. These results indicated that the anticonvulsion of EORTCD might be related to fatty amides.Monoamine neurone transmitters and their metabolites of CNS was assayed by high performance liquid chromatography with electrochemical detector. It was found that the content of NA was the highest, DA was the lowest and NA was 31 times higher than that of E in spinal cord; DA is the highest and E is the lowest in brain. Strychnine decreased 5-HT, NA, HAV of spinal cord, accelerated clearance of 5-HT and inhibited transition of DA in spinal cord. Strychnine also inhibited transition in brain. EORTCD could oppose the decrease , restore DA metabolic model and recover 5-HT level to the normal.The anticonvulsion effect of EORTCD (1.4g/kg) was vanished after reserpine or ondansetron pretreatment. However, the anticonvulsion effect of EORTCD.in higher dose could not be blocked by reserpine or ondansetronpretreatment. These results suggested that there were several ways for EORTCD to exert an anticonvulsion. The anticonvulsion effect of EORTCD was not affected by phentolamine or propranol. The results suggested that the anticonvulsion effect of EORTCD maybe chiefly mediated by 5-HT of neurone system.Conclusion: EORTCD showed an inhibition action on CNS and potent anticonvulsion effect on the model induced by strychnine. The process was related to the serotonin neuron system in spinal cord. |
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