Expression Of Caspase-3 And Bcl-2 In Transitional Cell Carcinoma Of Urinary Bladder

Objective: To study the expression of proto-oncogenc be 1-2 and caspase-3 in transitional cell carcinoma of bladder and to evaluate their significance on the biological behavior of the bladder neoplasm. Methods: In stiu hybridization was used to determine caspase-3 and bcl-2 expression in transitional cell cancer of bladder and normal bladder mucose. Results: the positive expression of caspase-3 was seen in 3-1.2% (12/35) tumors. Adjacent normal bladder muose showed more caspase-3 positivity (5/5) as compared with transitional cell cancer of bladder (P<0.05). Twenty-eight of 35 (80%) cases of transitional cell cancer of bladder were found to express be I~2 while only one of five (20%) cases of normal bladder mucose expressed positive. There was statistically significance between them (p<0.05). With increasing grade of transitional cell cancer of bladder, the positive expression of caspase-3 was descended while the positive rate of bcl-2 was increased gradual 1 y. 15 u t there did not have statistical significance between the different grades. There was mutual effect between theincreasing expression of hcl-2 and the decreasing expression of caspase-3 in transitional coll cancer of bladder (P<0.05) . Conclusion: over-expression of be 1-2 and under-expression of caspase-3 had been found in transitional cell cancer of bladder. It is very possible that proto-oncogene be 1-2 reduces the apoptos i s of tumor cell by restrained the action of caspas-3 in early bladder cancer.