Hairy-root Culture Of Polygonium Multiflorum And The Identification Of Its Active Constituents--anthraquinones

Transformed hairy roots of Polygonium multzflorum Thunb. were obtained by the infection of two kinds of Agrobacterium rhizogenes strains LBA9402 and Rl601 and an in vitro culture system of the hairy roots was established. It was clearly demonstrated that T-DNA of A. rhizogenes Ri plasmid was integrated into the hairy roots from LBA9402 by the experiments of PCR, Southern hybridization and paper electrophoresis. The transformed roots from strain R1601 was identified by paper electrophoresis. It was also showed that there were some significant characteristics of hairy roots after eliminating bacteria, such as more branches and black growth tips, fast growth, genetic and biochemical stability, and growth in hormone-free media. As a result, they showed a 11.03-fold increase in biomass in MS medium after 40-days of incubation. The compounds of anthraquinones 5 THESIS FOR MASTER DEGREE ABSTRACT (emodin , chrysophanol and rhein) from cultivated hairy roots were identified by means of HPLC and TLC, and the content of rhein was 2.85 folds higher in hairy roots than that of natural plants.