Rabbit Articular Chondrocyte Apoptosis Induced By Nitric Oxide

Objective : To investigate the effects of nitric oxide (NO) on using tissue engineered cartilage to repair cartilage defect, in terms of apoptosis aspect. Methods: Rabbit articular chondrocytes apoptosis was induced by NO which was generated from sodium nitroprusside (3mmol/L and 6mmol/L) . After administration of NO, at 4, 8, 12, 16, 20h, chondrocytes apoptosis was observed by using electron microscopy, FAGS analysis and TUNEL staining. Results: chondrocytes Apoptosis was induced by sodium nitroprusside (3mmol/L), with the extend of the time, nucleuses appearing from gathering in edge to distraction were observed by electron microscopy. Chondrocytes apoptosis percentage was 12. 71 汕头大学医学院硕士研究生学位论文 士3．267at 12h,叩optosis percentage was 32．94士5．48％at 16 h, andupto93．42土4．12％at20h,whlchwereh工her(hanthose In normal controls ( 士0．32％),the difference。as very slgnlflcant (P ＜0．01),by FACS analss。s；Chondrocytes poptos。s percentage ．was 10．27土3．12yat 12h,poptosis percentage was 20．16土6丁45％ at 16 h,and up to 70．36士12．71％at 20h,which were higher than those in nor。al controls(OIO．477),the difference。as very significant(P ＜0．01),by T洲EL stalnins．S。ollen necrotlc chondrocytes were observed In condition of addingsodium nltroprusslde in 6llllllol／L． Conclusion:High levels ofN0附v Induce aooptosis in culturedrabblt artlcular chondrocytes,wlthapoptosls percentage up topeakat 20h．Chondrocytes apoptosls was concentratedapoptosls,wlthoutdlffusedistribution．Veryhigh levels ofN0may cause chondrocytes necroses一The abnormal Increase ofchondrocytes即optosls percentage Is one ofreasons for disturbing tlssueenglneered cartilage to repair cartilage defect．...