The Expression Of Insulin-like Growth Factor-II In Human Uterine Endometrium In Proliferative Phase

The human endometrium undergoes proliferative and secretive changes periodically in order to prepare for embryo implantation. Traditionally, it was considered that the periodical chang of endometrium is mainly controlled by steroid hormones. Recently, many studies have proved that the proliferative and defferentiative action of steroid hormones to endometrium is modulated and mediated by growth factors and its relative peptides. Insulin-like growth factors (IGFs) is one of those growth factors which is now mainly focused on and studied. Similarly to insulin in structure and function, IGFs are cell mitogens and have an important modulate role to cell proliferation and differentiation. There are intact IGFs system in human endometrium, and its expression in endometrium has the temporal and spatial character. Although the locally expressed insulin-like growth factors have an important role to modulate the endometrial proliferation and differentiation via autocrine and/or paracrineway, there are only few studies to it. As to IGF- II, the present studies are mainly focused on its expression and roles in midlater secretory phase. It is thought that IGF- II may play possible roles in promoting endometrial stromal cell decidualization, modulating syncytiotrophoblasts nutrition transportation, placental hormone synthesis/secretation, and maternal-fetal cell-to-cell communication. But-4-2001~4~-i~Y.there are few studies about the expression and roles of IGF- II in endometrial proliferative phase.It has been proved by hybridization in situ that there are IGF- II mRNA expression in endometrial proliferative phase. As an insulin-like growth factor, IGF- II is a cell mitogen, suggesting a potential role for IGF- II in promoting proliferative endometrium growth. Investigation the expression and roles of IGF- II in proliferative endometrium , will not only helps to understand the physiological changes in endometrium, but also helps to explore the pathogenesis of endonietrial diseases.Objection:Using immunohistochemistry method, to test whether there is a positive staining of IGF- II in proliferative endometrium. Investigation the relationship between IGF- II, ER, PR and steroid hormone levels, analysis the role of IGF- II in proliferative endometrium.Method:twenty-six proliferative endometrial biopsies were obtained from regularly cycling women and grouped into three according to the cycling day( group I : 6-8day, group II: 9-llday, grouplll: 12l4day ). Immunohistochemical staining intensity of each antigen (IGFII, ER , PR )using the semi-quantitative grading system (HSCQRE) were analysised. Circulating E2, P levels were also analysised.Result:There was a positive staining throughout proliferative phase in endometrium, majorly stained in gland epithelium. The positive stain localized in cytoplasma, whereas in stroma cells, IGF- II staining is weak. The IGF- II staining is gradually increased throughout the proliferative phase, with a significant difference between group I and group III(p