Study On The Polymorphisms In GH And GHR Gene And Their Relationship With Partial Body Measurement Traits In Qinchuan Cattle

A 502 Qinchuan Cattles (Bos tarurs) population aged 2 years were selected to detect polymorphisms in exon 5 of GH gene by using PCR-RFLP and in GHR gene by using PCR-SSCP,and to study their associations with Qinchuan cattle partial body measurement traits.The aim was to explore genetic marking loci in GH and GHR gene which had association with partial body measurement traits in Qinchuan cattle,and to provide the basis for selection of growth performance of Qinchuan cattle.The results of the research were as follows:1. Polymorphisms in GH-P1 locus and their association with partial body measurement traits in Qinchuan cattleThe research was conducted to detect SNPs in GH gene P1 locus of Qinchuan cattle. Results showed that a C→G mutation was founded in the 4th base in exon 5 of GH gene ,which led the amino acid changing from Leu to Val. There were 3 genotypes named AA,AB and BB respectively after digestion by AluⅠ.Allele frequency of A and B were 0.825 7 and 0.174 3 respectively.Χ2 test showed that the locus was not in Hardy -Weinberg equlibrium in the Qinchuan cattle population detected. Correlation analysis showed: individuals with different genotypes had significant differences in wither height, heart girth,body length and rump length.2. Polymorphisms in GH-P2 locus and their association with partial body measurement traits in Qinchuan cattle.The study was to detect SNPs in GH gene P2 locus of Qinchuan cattle.The result showed that a C→T mutation was founded in the 93th base in exon 5 of GH gene,which was synonymous mutation. There were 2 genotypes named CC and CD respectively, after digestion by AflⅡ.Allele frequency of C and D were 0.902 4 and 0.097 6 respectively.Χ2 test showed that the locus wasn't in Hardy-Weinberg equlibrium in the Qinchuan cattle population detected.Correlation analysis showed: individuals with different genotypes had significant differences in hip width,ischium width and rump length. 3. Combined genotypes in P1 and P2 locus in GH exon 5 and their relationship with partial body measurement traits in Qinchuan cattleThe result of analysis of two loci combined genotypes in exon 5 showed that body measurement traits of combined genotype ABCD in exon 5 were higher than AB and CD genotype and other kinds of combinations genotype.The effects of multi-locus combinations of marker were higher than those of single-marker locus.Combined genotype ABCD may be the best genotype combination in affecting body measurement traits of Qinchuan cattle.4. Polymorphisms in GHR-8 locus of GHR gene and their association with partial body measurement traits in Qinchuan cattleThe study was to detect SNPs in GHR-8 locus of Qinchuan cattle.The result showed that a T→A mutation was founded in the 4962th base in GHR gene ,which led the amino acid changing from Phe to Tyr. There were 3 genotypes named EE,EF and FF respectively after PCR-SSCP.Allele frequency of E and F were 0.610 6 and 0.389 4 respectively.Χ2 test showed the locus was not in Hardy -Weinberg equlibrium in the Qinchuan cattle population detected. Correlation analysis showed: individuals with different genotypes had significant differences in heart girth.In the other body measurement traits , individuals with EF and FF genotypes were higher than the EE genotype ,but no significant difference.