Genetic Diversity And Phylogeny Of Rhizobia Isolated From Root Nodules Of Soybean In Parts Of China

The genetic diversity and phylogeny of 29 soybean rhizobium strains isolated from parts of China,together with 13 reference strains,was analyzed by ERIC-PCR, 16S rDNA PCR-RFLP,GSâ…¡PCR-RFLP.The phylogeny of the representative strains were performed by 16S rDNA and GSâ…¡sequences analysis.The results showed that ERIC-PCR divided 29 strains into 7 groups at 82% level of similarity.The result of 16S rDNA PCR-RFLP divided 29 soybean strains into 7 groups at the 82%similarity,and they located in 3 phylogentic branches - genus of Rhizobium, Sinorhizobium and Agrobacterium.One group consisting of 13 strains was heredity difference with recent reference strain R.etli CFN 42~T.GSâ…¡PCR-RFLP divided all of 29 into 10 groups at the 83%level of similarity.The 16S rDNA sequencing analysis divided thaese 29 strains into 3 branches-Rhizobium, Sinorhizobium and Agrobacterium.The similarities between those strains located in Rhizobium and R.sullae USDA 4950~T was 99.0%;The similarities between CCBAU10109 and S.freii ATCC 35423~T was 100%;The similarities between CCBAU31002,CCBAU21024 and A.tumefaciense NCPPB 2437~T were 99.6%;The similarity between CCBAU01040 and A.rubi IFO 13261~T was 98.4%.The GSâ…¡sequence analysis was similar to that of the 16S rDNA sequencing, and divided the 29 strains into 2 phylogenic branches -Rhizobium- Agrobacterium and Sinorhizobium.The similarities between those strains located in Rhizobium and R.leguminosarum USDA 2370~T were at the 90.0%similarity;The similarities between CCBAU10109 and S.freii USDA 205~T was 99.5%;The similarities between CCBAU31002,CCBAU21024,CCBAU01040 and A.tumefaciense CCBAU 61139~T were 94.0%,93.9%and 95.7%.