The T-rflp Analysis Of Bacterial Diversity Of Mangrove Root Soils In Beihai And The Filtering And Identification Of Actinomycetes

Mangroves are the highly concentrated biodiversity coastal eco-critical areas which grown in tropical and subtropical gulf estuarine intertidal zone where under he influence of periodic tidal flooding submerged.In this paper, the microbial diversity in mangrove roots soil has been studied in tow aspects. The fist aspect are contain:measure the Sulfide, particle size, carbon, nitrogen and phosphorus content, salinity, pH of the soil sample in different Soil depth. The results indicate that: the proportions of sand, fine sand and very fine sand are more than70%in all samples. The soil in20-25cm soil depth has the lowest pH, salinity, sulfide, total organic matter and total nitrogen. The T-RFLP analysis of bacterial diversity of the total DNA in soil samples show that:the sample in0-5cm soil depth have121OTU, in6-10cm have106OTU, in11-15cm have53OTU, in16-20cm have128OTU, in21-25cm have148OTU, in26-30cm have151OTU, in31-35cm have172OTU, in36-40cm have133OTU, in41-45have190OTU, in46-50have190OTU. The results show that:the embedded in the soil of0-50cm soil depth. The second aspect are to processing nine sample of mangrove roots soil of Beihai using DDC pretreatment technology, Selective isolation for actinomycetes using16different medium of separation, extracting the actinomycetes bacteria DNA using the microwave method,16S rDNA sequence analysis of actinomycetes.72actinomycetes were been discovered respectively belonging to Microbacterium, Clavibacter, Curtobacterium, Cellulosimicrobium, Acrtobacterium, which accounted for91%of rare actinomycetes.These provide experimental basis for the research of soil microbial resourcesin the area of mangrove roots soil.