Evaluation Of Genetic Diversity Among Several Duck Breeds By Using Microsatellites

The genetic diversity of 32 microsatellite loci were analyzed with the microsatellite marker technology in(?)veral duck breeds(Jinding Duck,Liancheng white Duck,Putian black Duck and Shanma Duck, Peking Duck,Muscovy,Kangbeier Duck).By means of the allele frequencies,polymorphism information content(PIC),heteroygosity(H),effective number of alleles,genetic distance and results of dendrogram, the genetic variability and population structure in duck breeds were estimated.The results were as follows:1.For the 32 microsatellite loci examined,the total number of alleles was 371 in 7 breeds,and the mean allelic number of per locus was 11.7188.8 alleles were owned in every breed,every population has its own alleles and every locus has the dominant allele.2.The mean PIC of 32 microsatellite loci in 7 indigenous duck breeds was 0.5224.Mean PIC of CAUD070 was the highest(0.8159),some loci display singlet state in some breeds.The average efficient allelic number was between 5.1410 and 6.9611,mean efficient allelic number of Kangbeier Duck was lowest(5.1410)and that of Peking Duck was highest(6.9611).The mean heterozygosisty was from 0.5115 to 0.7000,which showed that the seven breeds had higher heterozygosisty.3.The Hardy-Weinberg equilibrium analysis revealed that there only little loci were equilibrium,such as APH13 and CAUD045 in Peking Duck,remarkable genetic disequilibrium was showed at most of the lo(?)7 duck breeds.4.The F-statistic analysis indicated that the F_STof different locus varied from 0.0381(inCAUD037)to 0.6277(in APL82),and the average was 0.2888,The Nm was from 0.3259(in APL83)to 2.5919 (inCAUD070),the mean was0.6156.5.The Nei's standard genetic distance D_S and D_A genetic distance among the seven duck breeds were estimated with the Popgene32 software.The dendrograms were drawed with upper two genetic distances.The reaults showed that the genetic distance between Liancheng white duck and Putian black duck was the closest,and that between Liancheng white duck and Muscovy was the most distant among all breeds.7 indigenous duck breeds were clustered into 3 groups based on the results of dendrogram.In the demdrogram,the first group included the four Fujian indigenous duck breeds (Liancheng white duck,Putian black duck,Jinding duck,hanma duck and Kangbeier duck);the second group included Peking duck;the third group included Muscovy.The reaults showed that the dendrogram of D_A and D_S genetic distance was similar with the real relation of 7 duck breeds.6.The results would be offered some reference for studying Fujian indigenous duck breeds resources.