| To understand the molecular characteristics of rabies virus isolates from Guangxi, we amplified the P gene and M gene of six isolates GXNN2, GXPL, GXPXD, GXQZD, GXYZD and GXNND, by RT-PCR with primers NSM1/NSM2. The amplified cDNA fragments were then cloned and sequenced.The P and M gene ORF nucleotide sequences of twenty-nine isolates, including twenty-three isolates isolated previously in our laboratory and other Sixteen isolates from China and abroad were compared. Whatever used the P gene or the M gene to construct the phylogenetic tree, both showed that rabies virus isolates from Guangxi could be divided into three groups. Seventeen isolates GXHX82, GXHXB, GXLC9, GXQZD, GX08, GXNND, GXHX, GX014, GX195, GXLB, GXSL, GXWXp, GX260, GX01, GX09, GX091 and GXLA belong to groupâ… . Eleven isolates GXLCC, GXPL, GXYZD, GXBM, GX219, GX304, GXLA11, GXNN2, GX074, GXPX and GXPXD belong to groupâ…¡. GXN119 isolate belongs to groupâ…¢Six isolates in this experiment, two isolates GXQZD and GXNND belong to groupâ… , four isolates GXPL, GXYZD, GXNN2 and GXPXD belong to groupâ…¡.The homology analysis of the sequences of nucleotide and their deduced amino acid indicates that of P gene among the six isolates in this experiment range from 85.8% to 99.6% and 91.2% to 99.7, respectively. That of M gene range from 89.7% to 100% and 96% to 100%, respectively. That of P gene between the six isolates and twenty-three isolates in our laboratory range from 85% to 100% and 90.2% to 100%, respectively. That of M gene range from 88.5% to 100% and 95.5% to 100%, respectively.Additionally, we found that P gene amino acid specific mutation sites in group I are 69, 70, 130 and 131, in group II are 134, 135, 140 and 151, in groupâ…¢are 73,162,167,174, 240, 253 and 281, the 90 amino acid sites is specific for the group I andâ…¡, while the 57, 63 and 241 are specific for the groupâ… ,â…¡andâ…¢. M gene amino acid specific mutation sites in group II is 17 and 20. |
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