Mitochondrial Genome Comparison Research Of Soybean Cytoplasmic Male Sterility

Cytoplasmic male sterility (CMS) in plant is a common phenomenon of fail to produce functional pollen in the course of sexual propagation. It is a frequently encountered phenotype in flowering plants. Since 1921 CMS was first found in flax, and so far, it has been found in maize, broomcorn, rice, wheat, rape and sunflower. And the phenomenon is more effective in the using of heterosis.Great success has been achieved in the development of hybrid varieties in several major crops, including rice and maize. Due to lack of genetic male sterile resources or mutants, soybean is one of a few crop species, in which heterosis has not been exploited to a extent that is practically viable until recently. Huan Sun et al. found the first soybean CMS line in 1993 and great progress of studying and using was made in this field. However, little has been known about its molecule mechanism until now.One pairs cytoplasmic male sterile lines (JLCMS1A) and their maintainer lines (JLCMS1B)are used in this experiment. We design 39 probes to southern blot with sterile lines and their maintainer lines and compare to analysis to study the molecule mechanism of cytoplasmic male sterility of soybean.The main results are as follows:1 .Mitochondrial genome of soybean sterile lines is 375kb, encoding 31 proteins, 17tRNA and 3rRNA.2. Eighth of thirty-nine probes which used to southern blot between sterile lines and their maintainer lines have different signal. Besides, sixth of seven sterility relative gene were detected difference signal. These gene were atp9,coxII,coxIII,cob,atp6,atpA,coxI.3. Two Cytochrome B genes were isolated from mitochondrial DNA of JLCMS1A and JLCMS1B, encoding a same protein consisting 394 amino acids.4. A steady and high-efficient reaction system of molecular blot has been set up in this study and creates a basis for further study.