Intervention Effect And Difference Of Invigorating Spleen And Invigorating Phlegm On Airway Mucous Hyperplasia In COPD Rats

COPD (chronic obstructive pulmonary disease) has the following characteristics:airway obstruction can partly reversible. airway high reation. chronic and progressive development.it is closely related to the nonspecific inflammation which due to that the airway or lung tissue are contacted with the harmful gas or particles. because of its high morbidity and mortality,COPD has become a hot spot in the global medical. through the literature theory and clinical research,with "airway mucus hypersecretion" "phlegm" theory as the breakthrough point,pure smoked COPD rats model is set up,by histopathologic observation,pulmonary function and blood gas analysis,enzyme-linked immunosorbent assay methods,from the perspective of inflammatory cytokines, this topic is main in observing the role and differences of Jianpiyifeihuatanfang and it delimotion to the COPD model of rat lung tissue. preliminary discuss the mechanism of COPD on Jianpiyifeihuatan methods. To provide objective evidence for the clinical application of Jianpiyifeihuatanfang. this total is divided into two parts:Part Ⅰ:SummaryThis part mainly elaborated the progress of COPD on the modern medicine and traditional Chinese medicine research. in modern medicine,there are much etiology or pathogenesis theories of COPD, through combing we known, goblet cells hyperplasia and airway mucus hyper-secretion are the important pathogenesis of COPD. but there is no enough attention to it in academic circles. the traditional Chinese medicine has a clearly understanding on COPD. In the name of disease, etiology, pathogenesis, pathological nature,pathogenic products to analyze. we are realize "phlegm" are a major factor in the pathogenesis of COPD. "phlegm" go through the whole progress of COPD. from the point of syndrome classification and corresponding treatment,and treating outward sign and source of disease together,traditional Chinese medicine can effectively prevent and treat the COPD.Part Ⅱ:Trial processingObjective:To esplore the significance of "inhibition of mucus hypersecretion" or "phlegm" on treating COPD. we established model rats of COPD using pure smoked method and choosed Jianpiyifeihuatanfang and its separated parts as administration drugs. the subjects were divided into five groups:blank group, model group, jianpiyifei group, huatan group, jianpiyifeihuatan group. Firs t,by detecting the pulmonary function tests and arterial blood gas analysis and using light or electron microscopy to observe the pathological tissue slice,we can see the lung ventilation and pathological morphological change s of model rats of COPD, so we know the improvement、prevention and cure fun ction and curative effects which the Jianpiyifeihuatanfang and its separate d parts to the model rats of COPD. Second, to observe the goblet cells hype rplasia of lung tissue of COPD rats, and the content change of inflammatory cytokines just as IL-13、IL-1β、COX2、TNF-α、TGF-α、PGE2 and the mucins o f MUC5AC through the AB-PAS and ELISA methods. we can futher analysis the a djust action of Jianpiyifeihuatanfang and its separated parts to model rats of COPD. The purpose is to explore the mechanism of Jianpiyifeihuatan to g oblet cells hyperplasia and mucus hypersecretion in airway of the model rat s of COPD from the angle of inflammatory cytokines.Method:The model rats of COPD were established by pure given cigarettes fume atmosphere. After that, all the subjects were randomly divided into six groups including blank group, model group, jianpiyifei group, huatan group, jianpiyifeihuatan group and Carbocisteine group. Jianpiyifeihuatan and its separated parts were chosen as the administration drugs and the blank group was administration with pure water as well as model group. The dosage of drugs and pure water was equivalent and the time zone was 4 weeks. HE staining was used to find the morphological changes of the lung of rats by observing under optical and electron microscope. The pulmonary function tests and arterial blood gas analysis were taken to find the lung ventilation and the blood flow changes. AB-PAS and ELISA methods were separated to find the level of goblet cells and meture the content of mucins of MUC5AC and the inflammatory cytokines of IL-13、 IL-1β、COX2、TNF-α、TGF-α、PGE2. To observe the function and difference of Jianpiyifeihuatan and its separated parts to the model rats of COPD by using the above methods.Result:Pathological observation by HE staining under optical and electron microscope:the results of observation under optical microscope and electron microscope showed that blank group had no abnormal conditions about lung tissues; compared with the blank group,the lung tissues of COPD rats had the abnormal conditions:the cilia fell off, the inflammatory cells infiltrationed and secretions increased; compared with the model group, the above pathological changes of the lung tissues had been improved including Jianpiyifei group, huatan group, jianpiyifeihuatan group, especially the Jianpiyifeihuatan group.Lung function and Aterial blood gas analysis:compared with the blank group, the relevant indicators were abnormal conditions in model group:the level of FEV0.3/FVC、pH、PaO2、SaO2 were decreased, the PaCO2 was increased; compared with the model group, the above relevant indicators had been improved including Jianpiyifei group, huatan group, jianpiyifeihuatan group, especially the Jianpiyifeihuatan group.AB-PAS and ELISA:the goblet cells had no hyperplasia and the mucins of MUC5AC were narmal as well as the relevant inflammatory cytokines of IL-13、IL-1β、 COX2、TNF-α、TGF-α、PGE2 in blank group; compared with the blank group, the goblet cells were hyperplasia and the content of MUC5AC、IL-13、IL-1β、COX2、TNF-α、TGF-α、PGE2 was increased. compared with the model group, the above changes had been improved including Jianpiyifei group, huatan group, jianpiyifeihuatan group, especially the Jianpiyifeihuatan group.Conclusion:In summary, Jianpiyifeihuatanfang and its separated parts could decrease the content of inflammatory cytokines, so as to suppress the goblet cells metaplasia and mucus hypersecretion, to improve the lung function and Arterial blood gas and the pathological conditions of lung tissues of model rats of COPD. but there are some differences among three Traditional Chinese Medicine group:the best is Jianpiyifeihuatan group, then group is Jianpiyifei group, last group is the huatan group.