Primary Study Of Ion Beam Bio-technology In Revers Genic Sterile Rice Improvement

In search of new rice idioplasm resource was cried for the research of two-line hybrid rice according to the techno-thought of "revers genic sterile rice" , so that the potential risk of two-line hybrid rice seed production could be eliminated radically. Revers genic sterile rice was choosed from the offsprings of 99-02 by low energy N~+ ion beam, the growth potential and plant types of which needed further improvement. In the research, low energy N~+ ion beam was used as inducing sources; five diploid rice materials, revers genic sterile rice etc., were used as acceptor; maize was used as DNA donor. The mutants werc choosed from the rices that were mediated by low energy N~+ ion beam, and the character of mutants were studied. At the same time, the high frequency regeneration system of nonglutinous rice mature embryo was established in order to choose mutants quickly. The results are as follows.1. The biology effect which created by low energy N~+ ion beam was obvious. The rate of budbreak were different from dealing with different mode and doses. Two mutants were obtained that were induced by low energy N~+ ion beam, and their growing season were postponed. Six mutants were obtained that were mediated by low energy N~+ ion beam, the growth potential and tillering of mutants were strong, and their growing season were also postponed.2. Several mutants were choosed, and their physiological and biochemical index were mensurated in order to study the basis of biochemistry. Chlorophyll content were mensurated by ultraviolet absorption method, and the result showed that mutant plants were higher than control plants, and the chlorophyll content of one mutant plant from 99S was even 48.67% higher compared with the control. POD, CAT and SOD enzyme activity were respectively mensurated by guaiacol method, ultraviolet method and light deoxidized method, the results showed that three enzyme activities of mutant plants were different from control plants.3. The genome DNA of two mutant plants were respectively analyzed by 16...