| Four metabolizable trials were conducled to study the interactive effects of feed enzyme on the nutrient value of wheat, corn, wheat bran and soybean with 3 factors quadratic regression orthogonal design. The energy value and nutrient true availabilities were measured by the Sibhald method for measuring metabolizable energy. The 3 factors are non-starch polysaccharides enzyme, which corresponds to the feed ingredient containing the primary non-starch polysaccharicles. Wheat primarily contain xylan. B-glucan and cellulose, xylanase, B-glucanase and cellulase were added. Soybean meal primarily contain eel lulose, pectin and mannan, cellulase, pectinnase and mannanase were added. Corn primnrily contain xylan, cellulose and B-glucan, xylanase, cellulase and B glucanase were added. Wheat bran primarily contain cellulose, xylan and B-glucan, cellulase, xylanase and P -glucanase were added. Each enzyme given five levels (xylanase: 0,835, 3200, 5565,6400 U/kg;-glucanase:0,21,80, 139, 160U/kg; cellulase: 0,200, 800, 1392, 1600U/kg; pectinnase: 0,313, 1200, 2087, 2400U/kg; mannanase:0,522, 2000. 3478, 1000 U/kg). In each trial, Ihere are 18 treatments, among which 17 treatments were used for forced-fed, one treatment was used to measure endogenous nutrient by starvation. 102 healthy adult male Avian broilers were distributed randomly into 18 treatments with ti replicates of Ibird per replicate in each trial, the zero level group with 4 replicates of bird per replicate in each trial. The birds were forced-fed with the diet at the amount of 2% of body weight after 48hrs starvation period. Excreta was collected for 48hrs. The results and regression analysis are showed as follows:(1) Enzyme supplementation improved nutrient utilization and energy value of feed material of different kinds. Compared with the group without enzyme, NSP enzyme supplementation improved the energy value and true availabilities of nutrients in wheat, corn wheat bran and soybean meal to a certain exent. The optimal level of NSP enzyme improved the true availability of crude protein, dry matter and the AME AMEn TME TMFn value of wheat by 35. 56%, 8. 23% 21.02%, 17.16%, 17.98% 23.38% respectively. The true avai lability of crude protein, dry matter and the AME AMEn TME, TMFn value of soybean meal were improved by 35.56%, 8.23%, 21.02%, 17.46%, 17.08%, 23.38% respectively. The trueavai lability of dry matter and the AME AMEn TME, TMEn value of corn were improved by 4. 74%, 2. 28%, 4. 01%, 2. 18%, 3. 88% respectively. The true availabilily of crude protein, dry matter and the AME, AMEn, TME, TMEn value of wheat bran were improved by 64.88%, 10.60%, 9.48%, 5.34%, 8.46%, 6.46% respectively.(2)The influence of enzyme level on nutrient utilization is quadratic. By regressive analyses, the optimum combination and levels of NSP enzymes required depend on the kinds offeedstuffs. For wheat, the TMEn value was opt imized when xylanase, B glucanase, cellulase was added with 1998U, 112U, 1422U per kg respectively. For corn, TMEn value was optimized when xylanase, B-glucanase, cellulase was added with 3850U, 44U, 748U per kg respcetively. For wheat bran, TMEn value was optimized when xylanase, B-glucanase, eellulase was added with 4596U, 62U, 1039U per kg respectively. For soybean meal, TMEn value was opt imized when cellulase, pectinnase, mannanase was added with 171U, 2189U, 3778U per kg respectively. (3)The kinds of feed and levels of enzyme are dynamic variation related with nutrient value of enzyme. The optimal levels of different kinds of NSC enzyme combined properly, supplementation of the enzyme in the wheat, wheat bran, soybean meal, corn diet, the apparent energy value of enzyme is 1946. 90MJ, 689. 78MJ, 1491. 79 M.I, 291. 76MJ per ton respectively. With the change of enzyme additive level and enzyme combined, the apparent energy value of enzyme was different.This trial showed that enzyme supplementation improved energy value and true availabilities of nutrients in wheat, corn, wheat bran and soybean meal. The extent of improvement depends on... |
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