Studies On Optimum Protein To Energy Ratios Of Wheat Dietary Of Layer Added Non-starch Polysaccharides Enzyme

This experiment was conducted to study effects of different level of protein to energyratio on production performance, immune function, nutrients utilization, Cecal Microbe,serum biochemical indicator of hens under the conditions of adding non-starchpolysaccharides (NSP) enzyme to wheat meal diets.Using3*4two factors experiment design, select1440Hy-line laying hens280days ofage, which is divided into12groups, each group has4repeats. Set3levels of energy indiet:10.69MJ/kg、11.29MJ/kg、11.89MJ/kg,4protein levels:15.5%、16.5%、17.5%、18.5%,each group supplemented NSPE0.01%. Back-grounding7d, trial period70d, Resultsindicate:(1)The effect of different protein level on feed intake and average egg weight differenceswas significant (p<0.05), there were significant effect on egg weight with17.5%proteinlevel (P<0.05),18.5%protein levels dietary’s feed intake was the best, there was nosignificant effects on average laying rate, feed/egg ratio. There was no significant effectson production performance. Interaction effects of protein to energy ratio were remarkableon egg weight, laying rate and feed/egg ratio (P<0.05).(2)The effect of protein level of Day grain on haugh unit and egg yolk relatively heavywere remarkable (P<0.05), there were significant effect on haugh unit and egg yolkrelatively heavy with17.5%protein(P<0.05). The effect of energy level of Day grain onegg yolk relatively heavy and TC were remarkable(P<0.05), the egg yolk relatively heavywas the best in the treatment contained10.69MJ/kg which treatment was reduced onTC(P<0.05). Interaction effects of protein to energy ratio was remarkable on TC(P<0.05).(3)Protein levels effect the layers digestibility of DE, CP, Ca significantly(P<0.05),17.5%protein level dietary’s digestibility was the best(P<0.05). Energy levels effect thelayers digestibility of Ca significant(P<0.05),10.69MJ/kg energy level better than otherlevels significant(P<0.05). Interaction effects of protein to energy ratio was remarkable ondigestibility of Ca, the combination of17.5%protein level and10.69MJ/kg energy levelwas best in the digestibility of Ca.(4)Affection of protein levels of diet on thymus index and spleen index of laying henswere significant(P<0.05).17.5%and18.5%protein levels on thymus index were higherthan15.5%protein level(P<0.05), the spleen index was the highest in the treatment which contained18.5%protein in the diet(P<0.05). The affection of energy level on spleen indexof laying hens was remarkable(P<0.05),11.89MJ/kg energy level was better than othersremarkable(P<0.05), Interaction effects of protein to energy ratio was no remarkable onthymus index and spleen index of laying hens(P<0.05).(5)Protein levels effect levels of Newcastle antibody titer was remarkable(P<0.05),18.5%protein level higher0.3than15.5%. The effect of energy levels and protein toenergy ratio were no significant on Newcastle antibody titer.(6)There were no significant effects of different protein levels on Colibacillus,Lactobacillus and Bifidobacteria, Colibacillus on17.5%protein level was comparativelylower. There were no significant effects of different energy levels on Colibacillus,Lactobacillus and Bifidobacteria,10.69MJ/kg energy level was comparatively higher onLactobacillus, Bifidobacteria and the Colibacillus was negative correlation with energy.(7)There was significant effect of different protein level on serum UA, as proteinincreasing, serum UA increasing firstly and then falling trend, where the serum UA islowest under the conditions of the17.5%protein level. There was significant effect ofdifferent energy level on GLU and TC, GLU was higher than others when the level ofenergy was11.89MJ/kg,10.69MJ/kg energy level was the lowest on TC.The results showed that the optimum protein to energy ratios was16.37g/MJ of wheatdietary of layer added non-starch polysaccharides enzyme.