| The terminal buds and axillary buds and leaves were used for explants in the study of in vitro culture to establish a rapid technique system of micropropagation . Orthogoal design and SPSS for Windows statistical analysis software were employed in the experimentation. The results were as follows:1) Higher livability was obtained by stripping out squama, remaining two or three leaf anlage, sterilizing for five minutes in 0.1% Hgcl2 and some laundry powder, washing four or five times with asepsis water.2) Iniation medium: MS/2 (macroelement), MS (microelement), iron salts and organic substances with agar 0. 55%, sucrose 3%, 6-BA1. 5mg/l, IBA0. 1mg/l, GA31 -3mg/l was suitable for shoot growth and differentiation3)Multiplication culture: MS with agar 0.55%, sucrose 3%, 6-BA1.5 mg/1, IBAO. 2 mg/1 was suitable for multiplying buds. Theoretically, the rate of propagation of a shoot plumule could reach about 3 in 30-40 days and 5. 3x105 per year.4) Multiplication and the length of the stems were induced by adding some dose GA3. Multiplication coefficient could reach 6.47 and the length of stems could reach 3. 9cm by adding GA3 3. 0mg/l.5)Rooting:Roots were induced in MS/2+ agar 0.50%+sucrose 3%+IBAO. 5mg/l +NAAO. 3-0. 5mg/l+0. 1%AC, and the rooted plantlets were transferred into 1 /2MS.6)Leaf culture medium: NN69+ agar 0. 55%+sucrose 2% + 6 ?BA3. 0mg/1+ IBA0. 3mg/l, the rate of callus induction being 87.25%.7)Different lines of mother plants reacted differently. |
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