Study On The Key Technologies Of Cottonseed Dephenolization Protein And Refining Gossypol

In this paper,the processing of degreasing and phenol removal after the liquid - liquid - solid three-phase extraction method was studied.The cottonseed meal after extraction and the gossypol extraction by dilution with water from the ethanol phase were researched.The specific contents included:The processing conditions factors of affecting cottonseed oil removal from the cotton and kapok kernel were studied.The results showed that the best conditions of cottonseed oil removal were as follows:extraction temperature 55℃,extraction time 3h,the number of extracted 3 times,the liquid ratio 1:3.Under these conditions,cottonseed oil derived rate was 2%.The processing of gossypol removal from cottonseed meals was researched.The results showed that the optimum conditions of the phenol removal were as follows:extraction temperature 50℃,extraction time 1.5h,the number of extraction 3 times,the liquid ratio of 1:5.Under these conditions,free gossypol(FG) residues were 347mg/kg,combinative gossypol(BG) residues were 1827mg/kg in cottonseed meals.The quality of phenol protein of cottonseed was analysis.The phenol protein of cottonseed obtained through the liquid - liquid - solid three-phase extraction.The results showed that the main quality indicators of phenol protein of cottonseed through liquid - liquid - solid three-phase extraction were as follows:protein content≥50%,content of free gossypol (FG)≤400mg/kg,content of combinative gossypol(BG)≤2000mg/kg,content of fiber≤8%. The protein fully met and exceeded the requirements of national standards.It is a better quality protein.The gossypol extraction by dilution with water from the ethanol phase was researched. The results showed that gossypol was almost insoluble in alcohol peroxide solution of low concentrations.The gossypol was separated out through addition water to the ethanol leaching solution,and the better gossypol extraction conditions were found.The results showed that the gossypol extraction by dilution with water from the ethanol phase were as follows: temperature 25℃,holding time 6h,concentrated volume 40mL,the volume of water for concentrated volume 1.2 times.Under the above conditions,the production rate of gossypol was 23.143%,the purity was 92.276%.