The Primary Study On Identificating Biomarkers Of B[a]P Based On Chlamys Farreri

Polycyclic Aromatic Hydrocarbons are carcinogenic,teratogenic and mutagenic. They are the typical persisitent organic pollutants in nature.Because of development of maritime petroleum and marine traffic and industry sewerage discharge,polycyclic aromatic hydrocarbon(PAHs) pollution became seriously.Author studied the influence of B[α]P on the process of detoxifcation and metabolism,DNA and protein of Chlamys farreri.Then we adoped the statistical method to analyze the all the index we chosed,and applied them to the offing of Qingdao.The following results were obtained:1.The toxicological effect of PAHs on the tissue of C.farreri.We studied the index in gill and digestive gland of C.farreri exposed to B[α]P: inclued AHH,GST,GSH and MDA.The results indicate that index in all treatment groups were induced,and there's a time-course dependent character.In the first exposure period,AHH and MDA were induced significantly and showed a direct correlation with the concentration of B[α]P.The GST and GSH of the lower concentration group were elevated first and restrained to be the control level.While the higher concentration group were restrained at first.In the elimination period,the index in all the group resumed to the control level,and the lower concentration groups resumed quickly than the higher concentration groups.Also the digestive gland had a better resume capacity than gill of C.farreri.The index in the second exposed period showed a similar change with the first exposure period.2.The toxicological effect of PAHs on DNA and protein in gills and digestive glands of C.farreri.In this study,the DNA single strand breakage used two methods(Alkaline Unwinding Assay and Comet Assay),DNA-protin crosslink(DPC) and protein carbonyl content in gill and digestive gland of C.farreri exposed to B[α]P were exzamined.We found that all the index showed a significant time- course dependent character.In the firsr exposed period,DNA single strand breakage increased first and becomed restored because of the repair systems initiated,but the repair systems of the highest concentration group were restrained.While the DNA-protin crosslink and protein carbonyl content did not show any repair progress.In the elimination period, DNA-protin crosslink and protein carbonyl content recoverd slower than DNA single strand breaks.Only the lower concentration group recoverd to the control level.In the second exposure period,the index showed similar changes with the first exosure period.And the DNA-protin crosslink and protein carbonyl content were higher than the first exposed period.So contrast with the comet assay,alkaline unwinding assay was more suitable to assess the PAHs pollution.The damage to protein was harder to recover than enzyme and DNA,it could be a potential biomarker of PAHs pollution.3.Study on the biomarkers identification technology of B[α]P based on C.farreri.In this study,we used the statistic methods analized the dose-course relationship, correlation of two exposed period and the Pearson correlation coefficient of all the index.Results showed all the index were dose-dependent with the concentration of B[α]P.Except the GSH and MDA,other index showed a linearity relation between two exposed period.And the Pearson coefficient among all the index showed a extremely correlation.The Pearson coefficient among all the enzyme and the Pearson coefficient among DNA and protein damage were higher than it between enzyme and DNA,protein damage.Therefor,we chose AHH activity,GST activity,DNA single strand breaks,DNA-Protein crosslink and protein carbonyl content to be applied in the offing of Qingdao.4.The wild application and Assessment of B[α]P biomarkers pollution in Chlamys farreri and B[α]P content in seawater from offing of QingdaoSub-surface seawater and scallop(Chlamys farreri) samples at 3 sites(include Honsshiya,Taipjiao and NO.8 dock) in the offing of the Qingdao in Apr.30 and Jul. 30 of 2007,Benzo[α]Pyrene(B[α]P) content of seawater and toxicological index of gill and digestive gland of were analyzed.Result indicated the B[α]P in seawater was NO.8 dock＞Taipjiao＞Honsshiya,in indicidual site April＜July.AHH activity and DPC between Taipjiao and Honsshiya both showed no difference(P＞0.05).GST activity, DNA single strand breaks and protein carbonyl content indicated well corellation with the B[α]P in seawater.Well the mussels in NO.8 dock damaged most among the three sample sites.So,we chose DNA single strand breaks and protein carbonyl content as the biomarkers of PAHs pollution in marine environment,based on bivalve mussel.It is very important for security of aquatic food,protection of halobios and people health.