A Study On Analysing Organic Acids And Amines By Ion Chromatography

In recent years the ion chromatography develops rapidly. And it has been used widely. In the paper, the recent progress of ion chromatography has been reviewed first, including technology of the apparatus, column, suppressor, detect methods and new applications of ion chromatography. Relative references are 58. Then, an HPLC using indirect conductometric detection is proposed for the determination of the five amino acids needed by human body in soy and vinegar. The effects of the composition of the mobile phase, the temperature of the column on method sensitivity and separation were investigated. The limit of detection is between 2.9 and 3.2 μ g/mL and the recoveries of the amino acids are above 90% except for Val. In chapter 3, a liquid chromatographic method using indirect conductometric detection is proposed for the determination of m-nitroaniline, ethanolamine, aniline, p-toluidine and triethylamine. Derivatization is not required. The effect of composition of mobile phase including the organic solvent, different conducting species and the concentration of the chosen acid on detector response was studied. The column temperature that affected separation of the ingredients was also studied. The calibration graphs exhibit wide linear concentration ranges with correlation coefficients of better than 0.99 for all the components except for m-nitroaniline with correlation coefficients of 0.97. The detection limits are between 1 mg/L-19 mg/L. The method had been applied to determine the amines in polluted water with the recovery of 95.3%. In chapter 4, an ion exclusion chromatographic method is proposed for the determination of organic acids in apple vinegar. The effects of the composition of the mobile phase, the flow rate and the temperature of the column on method sensitivity and separation were investigated. Limits of detection are between 9.17 and 78.0 mg/L and recoveries of the organic acids are between 90% and 110% except for oxalic acid. Citric acid, DL-malic acid, succinic acid and acetic acid are the main existent organic acids. In chapter 5, single-drop microextraction was used to separate and concentrate2,4-dichlorophenol (2,4-DCP) and 2,6-dichlorophenol (2,6-DCP) in polluted water and then...