Studies On Combinability Of Nature And Flavor Of Traditional Chinese Medicine-herba Ephedrae

Purpose With the split and combined of traditional chinese medicine as the entry point,a new methodology and technology has been utilized owing to the study of the capability to be split and combined of Ephesus on the intervention of the diseases and syndrome,thus indicating the effective association of Chinese medicine-Ephesus for the theoretical study of herbs.Method The fever syndrome model animals and asthma model animals are taken as the carrier on the intervention of the capability to be split and combined of Ephesus,with intent to test the urine and blood samples,evaluating its function of intervention and exploring the properties and efficacy of traditional Chinese medicine through the overall metabolic network and the changes of biomarkers.Results1.The connection of the properties and efficacy of Ephedra intervening for heat syndrome modelAccording to the results of previous studies,to study compositionality of ephedra for the classic prescription MHT and MXSGT.To verify the non-crossing the split components and composite components of ephedra through HPLC and UPLC-MS techniques.To use PCA multivariate mathematical statistical analysis,it was further demonstrated they are non-crossing for the split components and composite components of ephedra.At the same time,by copying the yeast,2,4-nitro phenol,lipopolysaccharide induced fever syndrome rat model,and to give the split components and composite components of ephedra,the experimental results proved that MHD has the best antipyretic effect,the composite components have a certain intervention effect on fever model.Through immunohistochemical study,it was found that MXSGT and ephedra polysaccharide components have significant inhibition effect on NFKB-P65,p38-MAPK,TGF-B1,Other components also have certain inhibitory effects.2.The mechanism of Mahuang decoction intervening for yeast-induced pyrexia ratsCopy of yeast-induced fever syndrome animal model to macro signs and metabolomics approach to evaluation model,the model successfully reproduced;meantime,make sure the fever syndrome can be characterized by the occurrence and development positive ion mode of 19 potential biomarkers and negative ion mode of 10 potential biomarkers.In these 29 biomarkers,20 biomarkers’expressed were unregulated and 9 biomarkers’ expressed were down-regulated for biological analysis,respectively.They are relative to Pantothenate and CoA biosynthesis,Biotin metabolism,Histidine metabolism,Steroid hormone biosynthesis,Riboflavin metabolism.MHT intervention by fever syndrome model to the macro signs and metabolomics approach to evaluation,that has a good MHT Intervention;the 29 potential biomarker trends with a callback that MHT intervention fever syndrome dose-effect relationship may exist;affected the metabolic pathways,such as Pantothenate and CoA biosynthesis,Biotin metabolism,Histidine metabolism,Steroid hormone biosynthesis,Riboflavin metabolism.It suggests that MHT intervention fever syndrome may exist concentration-response relationship;the overall changes of metabolites show the effect of MHT intervention for fever syndrome.3.New mechanistic insights for MXSGT inhibiting action on OVA-induced asthmaSuccessfully copied the egg albumin induced animal models of asthma,with macro signs and metabonomics approach to evaluation model,show that model copy success;At the same time,can determine the characterization of fever syndrome occurrence and development 23 potential biomarkers,the study found that 23 potential biomarkers after building 10 ions express rised significantly and 13 ions expression significantly lowered.At the same time,the 23 potential biomarkers respectively with citric acid metabolic pathways,glycerol phospholipid metabolic pathways,alanine,glutamic acid and aspartic acid metabolic pathways,purine metabolic pathways and other metabolic associated.Using MXSGT intervention asthma models to macro signs and metabonomics method to evaluate,suggests MXSGT has better intervention effect;Ephedra soup of 23 potential biomarkers with a callback trend and affect the citric acid metabolic pathways,glycerin phospholipids metabolic pathways,alanine,glutamic acid,aspartic acid and metabolic pathways,purine metabolic pathways,suggesting asthma model MXSGT intervention may exist concentration-response relationship;From the group overall change and biological markers of characterization of metabolites MXSGT to asthma intervention effect.ConclusionThis topic using the theory of "cold fever" of traditional Chinese medicine,the compound Chinese medicine as the research object,ephedra soup to replicate the fever syndrome animal model induced by yeast as the carrier,with metabonomics technology and UPLC-MS analysis method,from the metabolites of disease and the group as a whole,the changes of biomarkers and the mechanism of action of the evidence ephedra soup,at the same time,with egg protein induced asthma model of animal as the carrier,in metabonomics technology and network pharmacology analysis method,from the disease and metabolite group as a whole,the changes of biomarkers and the mechanism of action of the evidence MHT,for the research of traditional Chinese medicine medicinal theory provides a new train of thought,method and technology,to reveal the scientific connotation of traditional Chinese medicine medicinal theory is of great significance.