Long Noncoding RNA H19、MINCR And CircularRNA FOXP1 Promoted Tumor Progression In Gallbladder Cancer

Objective Gallbladder carcinoma(GBC)is an aggressive neoplasm and have poor prognosis in patients.Recent studies found long non-coding RNA and circular RNA have emerged as important regulators in several cancers.The study aims to investigate the clinical significance and functional role of H19,MYC-induced long non-coding RNA(MINCR)and circRNA FOXP1(circFOXP1)in gallbladder cancer.Methods In the study,the quantitative real time polymerase chain reaction(qRT-PCR),western blot,Northern blot,Dual luciferase reporter,RNA immunoprecipitation(RIP)and RNA-pull down assays were used to detect the expression of H19,MINCR and circFOXP1 in gallbladder cancer tissues and in vitro effects on cell proliferation and invasion,and in vivo the nude mice xenograft tumor model was also performed.Results We demonstrated that long non-coding RNA H19 was up-regulated in the gallbladder cancer tissues compared with adjacent normal tissues,in vitro and in vivo assays results showed that increased H19 promoted cell proliferation and invasion by negatively regulating miR-342-3p targeting FOXM1;MINCR was higher in GBC tissues compared with adjacent normal tissues and increased MINCR expression promoted cell proliferation and invasion in vitro and in vivo by directlyinteracted with EZH2 or sponging to miR-26-5p targeting EZH2;circular RNA FOXP1(circFOXP1)was up-regulating in GBC tissues compared with adjacent normal tissues,higher circFOXP1 expression promoted cell proliferation,cell cycle progression,suppressed cell apoptosis in vitro and enhanced the tumor growth in vivo.Mechanically,we confirmed that circFOXP1 interacted with PTBP1,which promoted cell proliferation in GBC.Conclusion Our results demonstrated that long non-coding RNA H19,MINCR and circFOXP1 acted as oncogenes in GBC,inhibition of H19,MINCR and circFOXP1 expression could suppress tumor progression,which provided therapeutic targets of gallbladder cancer.