Functions And Translational Application Of Two Noncoding RNAs:miR-638 And MEG3

Accumulated studies demonstrated that noncoding RNAs play an important role in regulation of cell growth, apoptosis and various metabolic pathways. Noncoding RNAs can be grouoped into 3 categories: RNAs of less than 50 nt, RNAs of-50 nt to 500 nt, and RNAs of more than 500 nt. In the current study, miR-638 belongs to small noncoding RNAs which are less than 50nt, and MEG3 is one of long noncoding RNAs which are larger than 500nt.MicroRNAs are a kind of regulatory non-coding RNAs, which are involved in many physiological and pathological processes, such as embryonic development and tumorigenesis. In recent years, it is shown that the microRNAs are also invovled in the regulation of.Autophagy not only regulates cell growth, differentiation and homeostasis, but also plays an important role in the process of tumor development. We found that miR-638 promotes esophageal cancer cells and MCF-7 breast cancer cells by inhibiting expression of its target gene DACT3, In addition, miR-638 can significantly promote cell proliferation, migration and invasion of cancer cells. Our results indicated that miRNA-mediaded regulation of autophagy may be an important molecular event in tumor and development. We provide a new approach to clarify the pathogenesis of cancer and other diseases, and to explore new therapeutic strategies.An increasing number of studies have shown that long non-coding RNAs(IncRNAs) play a very important role in cancer biology and may have great potential in the treatment of cancers. In this study, we found that expression of MEG3 is low in cancer tissue compared to normal tissues. Moreover, MEG3 can inhibit tumor cell proliferation via regulating the expression of P53. We developed a liver-targeting cationic polymer PuPGEA2-.This biomaterials could package MEG3 and P53 plasmids and form a nano-particals[PuPGEA2/MEG3-,PuPGEA2/P53 or PuPGEA2/(MEG3+P53-)].These nano-particals can effectively inhibit the proliferation , migration and invasion of hepatoma cells. The results showed that lncRNAs could be used as a new target for treatment of liver cancer.