Material Foundation Research Antiviral Patchouli Oil

Obejctive:Observing the effect of patchouli oil against influenza virus, respiratory syncytial virus, adenovirus, Coxsackie virus and herpes simplex virus in vitro. Screening the sensitive viruses to research the antivirus action of the drug. Verify the antiviral effect of Patchouli oil on viral animal model in vivo. Investigate the antivirus effect of the tow main components of patchouli oil which are patchouli alcohol and patchoulenone to discussing the antiviral pharmacodynamic substance foundation and action principle of patchouli oi.Methods:Establish models of influenza virus (H1N1) infecting MDCK cell, respiratory syncytial virus (RSV) and adenovirus (ADV) infecting Hep-2cell, Coxsackie virus (CVB3) infecting Hela cell and herpes simplex virus (HSV-1) infecting Vero cell respectively. Use CPE and MTT as the observation and detection methods, to investigate the drug toxicity to the cells and the antiviral effect of different concentrations of patchouli oil, screeing the sensitive viruses to the drug. Researching the drug action based on the virus replication characteristic. Establish the viral infected mice model with the most sensitive virus according to intraperitoneal injection, observing the influences of the model general condition, survival rate, heart,liver,lung and kidney index, pathological sections and serum biochemical index by patchouli oil with intraperitoneal injection. Verifying the antiviral effect of patchouli oil in vivo. On the other hand, investigate the drug toxicity to the cells and the antiviral effect of different concentrations of patchouli alcohol and patchoulenone which are the main mate composition of patchouli oil, screeing the sensitive viruses, researching the drug action to virus. Using normal PCR and Real-time PCR to locate the target of drug action to virus. Explore the antiviral pharmacodynamic substance foundation of anti-virus and principle of patchouli oil.Results:The cytotoxicity of Patchouli oil, patchouli alcohol and patchoulenone to MDCK, Hep-2, Vero and Hela cells are basically identical. The TC50of Patchouli oil, patchouli alcohol and patchoulenone to the cells are0.101mg/ml,0.125mg/ml and0.071mg/ml, the TC0are0.092mg/ml,0.031mg/ml and0.063mg/ml. Patchouli oil, patchouli alcohol and patchoulenone could inhibit the H1N1, CVB3and ADV virus induced cell lesion in different degrees, there’s no inhibition or very low on RSV and no inhibition on HSV-1. The IC50of Patchouli oil, patchouli alcohol and patchoulenone to H1N1are0.088mg/ml,0.031mg/ml and0.063mg/ml,the therapeutic index (TI) is1.13,4.0and1.13; to CVB3are0.08mg/ml,0.063mg/ml and0.067mg/ml, TI are1.25,2.0and1.06; to ADV are0.084mg/ml,0.063mg/ml and0.063mg/ml,TI are1.20,4.0and1.13. Patchouli oil and patchouli alcohol are better than patchoulenone on the role of antivirus.Patchouli oil and patchouli alcohol on the direct action of the virus is superior to the anti-virus adsorption and antiviral biosynthesis. And in direct action, Patchouli oil on CVB3inhibition rate is higher than H1N1and ADV inhibition and patchouli alcohol on ADV inhibition rate is significantly higher than H1N1and CVB3inhibition under the same concentration. The result of PCR and Real-time PCR show that patchouli alcohol could damage the Hexon gene of ADV. The LD50of patchouli oil on mouse by intraperitoneal injection is4.441mg/g, the95%prediction interval is3.768,5.317mg/g. Patchouli oil has a certain therapeutic effect on viral myocarditis mice, the concentration group of0.096g/kg is higher than the positive drup group in the field of survival rate and cardiac remission, and has a certain protective effect on liver and kidney, can increase the SOD activity, decrease the MDA and TNF-α levels,slightly lower LDH and CK-MB level.Conclusion:In vitro antiviral experiment, patchouli oil show the ability of anti-Coxsackie virus, anti-adenovirus, anti-influenza A virus except anti-respiratory syncytial virus, the anti-Coxsackie virus effect is superior than others, meanwhile patchouli oil has a good antagonistic effect on Viral myocarditis mice model injected by CVB3in vivo, this means patchouli oil has antiviral effect in vivo and in vitro. Patchouli alcohol and patchoulenone which are the main monomer components of patchouli oil show antiviral effect, patchouli alcohol is better than patchoulenone. Patchouli alcohol is obviously in anti-adenovirus on the target of Hexon gene which is responsible for translation capsid protein of adenovirus.