| Purpose：Gastric cancer is a serious impact on people’s lives and health ofmalignant tumor, around the world each year there were more than one millionnew cancer cases, China accounted for42%, killed about800thousands, Chinaaccounted for35%, China was one of countries with the highest morbidity andmortality of gastric cancer, incidence and mortality rate was more than two timesof the world average level. The diagnosis and treatment of gastric cancer wasthe important content of medical attention. In clinical treatment, we found thatmost gastric cancer patients had spleen-qi deficiency syndromes. They often hadthe symptoms of fatigue,weakness,loss of appetite, poor body resistance, weightloss and other clinical manifestations. Gastric cancer could affect theascending function of spleen and transport function of stomach. The spleendeficiency also reduced the body’s resistance to disease. These aspects madeit difficult to control gastric cancer. The spleen deficiency and stomach cancerinfluence each other. Preliminary clinical studies have proved that theformation of "Yiqi Jianpi anticancer" principle had the effect of anti-cancerin the treatment of digestive system tumor. It can improve the symptoms, reducethe toxicity of chemotherapy. The theory of the spleen deficiency essence ofgastric cancer reflects this relationship. In clinical practice, Yiqi Jianpianticancer method plays an important role in the treatment of gastric cancer.Through the experimental study reveals the spleen deficiency essence of gastriccancer, the mechanisms of Yiqi Jianpi anticancer drugs has important theoreticaland practical significance. This study is based on the theory of the spleendeficiency essence of gastric cancer, establishing human gastric cancer SGC-7901model in nude mice. Treat the gastric cancer mouse model with the traditionalChinese medicine of Yiqi Jianpi and Yiqi Jianpi anti-cancer. Observe relevantindexes of spleen deficiency: mice weight, food intake, water intake, gastricresidual rate, intestinal propulsion, somatostatin, spleen coefficient, spleen protein kinase C (PKC) effect; the parameters related to tumor growth and tumorbody: epidermal growth factor (EGF), vascular endothelial growth factor (VEGF)expression of gastric cancer with deficiency of the spleen, revealing the spleendeficiency essence of gastric cancer and the mechanism of Yiqi Jianpi anti-cancerdrugs.Material and method：1. Choose human gastric carcinoma cell line SGC-7901, the experimental animalwas nude mice.2. Drugs: Chinese medicine Yiqi Jianpi include: Radix Pseudostellariae, Poria,Atractylodes, licorice root, pinellia, dried orange peel, Amomum villosum,costas. Anticancer medicine composition include: tulip, tuckahoe, ZhejiangFritillaria, Oldenlandia diffusa.3.To establish the model of human gastric cancer cell SGC-7901xenografts innude mice: the culture of human gastric cancer cell line SGC-7901in vitro,selection of tumor cells in logarithmic growth phase, nude mice inoculated with0.1ml tumor cells, the tumor will formate15days after inoculation.4. Administration: experiment animals were divided into blank group, model group,Yiqi Jianpi group, Yiqi Jianpi cancer group. There were10rats in each group.The blank control group were treated with normal saline; model group were givensaline, Yiqi Jianpi group were given Yiqi Jianpimedicine; Yiqi Jianpi anticancergroup were given Yiqi Jianpi anticancer medicine. The blank group and model group weretreated with normal saline for14days. The drug groups were given correspondingmedicine for14days.5. Indicators: The first part of the experiment was the experimental study YiqiJianpi anticancer effects on gastric cancer in mice by absorption digestion,the indexs: nude mice weight, food intake and water intake, gastric residualrate, intestinal propulsion rate, determination of the content of somatostatinin small intestine and in plasma by radioimmunoassay. The second study was theeffects of Yiqi Jianpi anticancer on the PKC of mice spleen, the indexes:expression of PKC protein was detected by Western blot method.The third part of the experiment was observation on gastric cancer tumor growth in mice andtumor EGF, VEGF expression, the index: tumor weight, inhibition rate of tumor,and tumor cell apoptosis was determined by TUNEL method, the expression of thetumor EGF, VEGF were determined by Western-blot method.Results：1．Amounts of weight gain: blank group was5.99±0.24g, significantly more thanthe other three groups (P <0.05), model group was2.50±0.27g, and Yiqi Jianpigroup was4.21±0.35g, Yiqi Jianpi anticancer group was4.62±0.40g, the otherthree groups were better than the model group (P <0.05). No statisticalsignificance of Yiqi Jianpi group and Yiqi Jianpi anticancer group was observed,but the latter was better than the former trend. In the aspect of food intakeand water intake, showed the same results with the weight change, blank groupwas the best, followed by Yiqi Jianpi anticancer group, Yiqi Jianpi group andmodel group, the other three groups were better than model group.2．gastric residual rate and intestinal propulsive rate: in the blank group,the gastric residual rate was31.2±11.4%and small intestinal propulsion ratewas65.4±9.6%, was the best in all groups, had the minimum gastric residualrate and the maximum small intestinal propulsion rate, there were significantdifference (P <0.05) compared with the other three groups, the difference ofYiqi Jianpi anticancer group and the blank group was minimum. In model group,the gastric residual rate was48.3±12.6%and small intestinal propulsion ratewas44.8±11.3%, was the worst in all groups, there were significant difference(P <0.05) compared with the other three groups. Yiqi Jianpi group, gastricresidual rate was39.7±11.2%, small intestinal propulsion rate was56.3±10.4%,Yiqi Jianpi anticancer group, gastric residual rate was35.5±10.7%, smallintestinal propulsion rate was60.1±10.2%, there was no difference between thetwo groups, but the Yiqi Jianpi anticancer group had a better trend to Yiqi Jianpigroup.3. Somatostatin. the amounts of somatostatin were lowest in the blank group,the small intestine and the plasma levels were143±32ng/g and84±32ng/L. the amounts of somatostatin were the highest In the model group, were182±51ng/gand125±39ng/L; In Yiqi Jianpi group were177±46ng/g and110±42ng/L. In YiqiJianpi anticancer group were160±42ng/g and102±38ng/L. There were obviousdifferences between groups and the blank group (P <0.05). compared with themodel there was significant difference between Yiqi Jianpi group and the modelgroup (P <0.05), there was significant difference between Yiqi Jianpi anticancergroup and the model group also(P <0.05). Compared with the Yiqi Jianpi group,the Yiqi Jianpi anticancer group showed a good trend, but without statisticalsignificance.4. Spleen coefficient: the spleen coefficient in blank group was1.17±0.12%,model group was0.71±0.24%, Yiqi Jianpi group was0.94±0.18%, Yiqi Jianpianticancer group was1.06±0.27%. Compared with the blank group, model groupand Yiqi Jianpi group were less than the blank group, there were statisticallysignificant (P <0.05); the Yiqi Jianpi anticancer group were little less thanthe blank group, but there was no significant difference between the two groups.Compared with the model group, spleen coefficient of the other three groups weresignificantly higher than model group, there were statistical significance (P<0.05).5. The expression of PKC in the spleen: blank group was0.557±0.028, other groupswere Yiqi Jianpi anticancer group0.509±0.010, Yiqi Jianpi group0.442±0.017,model group0.419±0.021. Compared with the blank group, in the model group,the expression of PKC decreased most significantly (P<0.01), In Jianpi Yiqi groupand Yiqi Jianpi anticancer group, the expression of PKC also decreased (P<0.01,P<0.05). Compared with the model group, the expression of PKC were higher inJianpi Yiqi group and Yiqi Jianpi anticancer group,there were statisticalsignificance (P<0.01), the expression of PKC of Yiqi Jianpi anticancer groupwas highest in the three groups, but still lower than the blank group.6.tumor weight and inhibition rate of tumor: the tumor weight of model group,Yiqi Jianpi group and Yiqi Jianpi anticancer group were3.82±0.73g,2.76±0.25gand2.44±0.37g, the tumor growth of the two groups were less than that in model group, there were statistical significance (P <0.05); the tumor weight of YiqiJianpi anticancer group was less than Yiqi Jianpi group, there was statisticallysignificant (P <0.05). The tumor inhibition rate of Yiqi Jianpi group amd YiqiJianpi anticancer group were28%,36%, there was obvious statisticalsignificance.7. The number of apoptotic cells: the number of apoptotic cells of model group,Yiqi Jianpi group and Yiqi Jianpi anticancer group were0.80±0.79,7.30±1.57and18±2.45, Compared with the model group, the Yiqi Jianpi group and the YiqiJianpi anticancer group were higher, there were statistically significant (P<0.01). Compared with the Yiqi Jianpi group, the number of apoptotic cells ofthe Yiqi Jianpi anticancer group, was significantly more than the former, therewere statistically significant difference (P <0.01).8. The expression of EGF, VEGF: model group, the expression of EGF, VEGF of modelwere the highest, the expression of Yiqi Jianpi groups were lower than the modelgroup, but there were no statistically significant; the expression of EGF, VEGFof Yiqi Jianpi anticancer group were significantly lower than that of the formertwo, there were statistically significant (P <0.05).Conclusion：1. Gastric cancer can affect the metabolic function of the spleen, the Yiqi JianpiChinese medicine can improve the spleen function, can strengthen gastricemptying and small intestinal propulsion, when the Yiqi Jianpi Chinese medicinewere used with the anticancer Chinese medicine together, the improve effect canbe more obvious.2. Gastric cancer can inhibit the growth of spleen, inhibit the expression of spleenPKC, application of Yiqi Jianpi anticancer treatment, this inhibitory effectwas weakened.3. Yiqi Jianpi anticancer treatment compared with the single Yiqi Jianpitreatment, the inhibitory effect on the tumor is more obvious, not only hadanticancer effects, but also can inhibit the expression of the EGF and VEGF,these were important genes related to tumor growth. 4. Gastric cancer can affect the function of the spleen, the application of YiqiJianpi anticancer treatment can achieve the dual goal of strengthening the spleenfunction and anticancer. Therefore, in clinical treatment, in the applicationof Yiqi Jianpi medicine, at the same time, the anticancer medicine should becombined, this combination principle embodies the combination of disease andsyndrome differentiation and treatment of TCM treatment philosophy, worthy offurther promotion. |
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