The Study On Antidepressant Effect And Mechanism Of Jieyu Qingxin Anshen Decoction

Objective: To observe the effects of Jieyu Qingxin Anshen decoction (JYQXASD)and explore it’s possible antidepressant mechanism.Methods:1. Force swimming test and tail suspension test.①Select40female KM mice with18-22g, and divide them into4groups randomly:control group, Positive group, JYQXASD-H group and JYQXASD-L group,10in eachgroup.②Do the Intragastric administration for the mice every day at08:00,0.4ml salinefor control group,0.4ml fluoxertine hydrochloride saline liquor for positive group and0.4ml different concentrations of JYQXASD for different JYQXASD groups.③Record the accumulative immovability time in the late4minutes of the mice by theforced swimming test in each group on the7th day, and in the late5minutes of the mice bythe tail suspension on the8th day.2. Effects of JYQXASD on the depression mice induced by reserpine.①Select50female KM mice with18-22g, and divide them into5groups randomly:control group, model group, positive group, JYQXASD-H group and JYQXASD-L group,10in each group.②Do the intragastric administration for the mice every day at08:00,0.4ml saline for control group and model group,0.4ml amitrptyline saline liquor forpositive group and0.4ml different concentrations of JYQXASD for JYQXASD-H groupand JYQXASD-L group.③After the intragastric administration for14days, give reserpineto induce depression symptoms, and observe the body temperature, eye ptosis andakinese of mice.3. Effects of JYQXASD on the depression mice made by unpredictable chronic stress.①Select50female KM mice with16-18g, and divide them into5groups randomly: control group, model group, positive group, JYQXASD-H group and JYQXASD-L group,10in each group.②Do the intragastric administration for the mice every day at08:00,0.4ml saline for control group and model group,0.4ml fluoxertine hydrochloride salineliquor for positive group and0.4ml different concentrations of JYQXASD for differentJYQXASD groups. Except for the control group, The KM mice were subjected to10different stressors for60days to create the depression mouse model.③At the end ofexperiments, mice were analyzed with depression-related behavioral test. The body weightof all mice was recorded during the experiment process. Mood state was evaluated bysaccharin preference test and open-field test. The changes of cognitive processing weretested through Morris water maze.4. Study on the mechanism of JYQXASD on depression mice induced by CUSAfter the behavior tests, all mice were decapitated, and brains were rapidly removed.The levers of5-HT, NA, DA and BDNF in hippocampus, CRH in hypothalamus, ACTH,IL-1βand IL-2in blood of depression mice was measured all by ELISA.Results:1. Different dose groups of JYQXASD reduced significantly the immobility time ofthe mice during the forced swimming test and the tail test (p0.05).2. Compared to the control mice, the reserpine induced eye ptosis and akinese of mice,and decreased body temperature significantly (p0.05). These behaviors could berecovered by administration of JYQXASD (p0.05).3. The chronic unpredictable stress led to the slow increase of the weight of the mice,the inhibition of the excitation and reactivity of mice in open-field test, and induced theweakened abilities of exploration and memory in Morris water maze as compared to thecontrol (p0.05). Compared to the control mice, the depression model mice consumedsignificantly less cane sugar (p0.05). These behaviors could be recovered byadministration of JYQXASD (p0.05).4.The level of5-HT, NA and DA in the hippocampus of depression mice was allsignificantly decreased as compared to the control(p0.05), and could be elevated bytreatment with JYQXASD(p0.05).5. Compared to the control group, the level of CRH in hypothalamus and ACTH inthe blood of depression mice was significantly increased (p0.05); And JYQXASD coulddecrease the level of CRH and ACTH(p0.05). 6. The level of L-1βand IL-2in the blood of depression mice was all significantlyincreased as compared to the control(p0.05), and JYQXASD could decrease the level ofIL-1βand IL-2(p0.05).7. The chronic unpredictable stress could decrease the level of BDNF in thehippocampus, as compared to thud control (p0.05), and JYQXASD could significantlyelevate the level of BDNF (p0.05).Conclusion: Jieyu Qingxin Anshen decoction could reduce the immobility time of themice in the forced swimming test and the tail test, at the same time could reverse the ptosis,akinesia, hypothermia induced by Reserpine. Furthermore, JYQXASD could reverse thesymptom of depression induced by the chronic unpredictable stress. Chronic psychologicalstress can injure function of hippocampus which can be declined by JYQXASD.Ourstudies indicated that JYQXASD can enhance the function of monoamine neurotransmitter,regulate HPA axis hypoglycemia, strengthen the immunity ability, and at the same timereverse the decline of the lever of BDNF.