The Influence Of Rhubarb, Rhizoma Coptidis And Their Extracts On Whole Genome Expression Of Liver In Excess Heat Syndrome Rat

Objective：To detect the change of hepatic gene expression profile in rats with excessheat syndrome using gene chip technology, to study the effects of herbs with cold propertysuch as rhubarb, Rhizoma coptidis and their extracts on the change of hepatic geneexpression profiling, to discover the material foundation of cold property of Chinesemedicine and to discuss the mechanism of it from the aspect of functional genomicsperspective.Methods：96SPF Wistar rats were divided into two batches to complete theexperiment. The first batch animals were divided into the blank control group, excess heatsyndrome group, Rhubarb decoction group, Rhubarb ethyl acetate extract group, Rhubarbn-butanol extract group, Rhubarb water extracts group. The Rhubarb decoction group andthe extracts of Rhubarb groups were given the corresponding liquid in accordance with the10ml/Kg, the excess heat syndrome group and the blank control group were given10ml/Kgdistilled water. The second batch animals were also divided into6groups. The Rhizomacoptidis decoction group and the extracts of Rhizoma coptidis groups were given thecorresponding liquid in accordance with the10ml/Kg, the excess heat syndrome group andthe blank control group were given10ml/Kg distilled water.The rats were given subcutaneous injection of2,4-dinitrophenol saline solution toreplicate the excess heat syndrome model,0.5h later giving medication treatment,2h,4h,6h point later, the rectal temperature of rats was measured. After the extraction of totalRNA from liver tissue, the gene clustering analysis and functional classification annotationwere carried on. We selected the differential expression genes, analyzed the genes bycluster and conducted the significant analysis on the genetic function of differential genes. A part of genes was selected to test the accuracy of results by RT-PCR.Results：There were167strips of differential expression genes in excess heatsyndrome group as compared to blank control group. There were177、139、287and177strips of differential expressions in Rhubarb ethyl acetate extract group, Rhubarbn-butanol extract group, Rhubarb water extracts group and Rhubarb decoction group,respectively compared with heat syndrome rat group.336、362、417and218strips ofdifferential expressions were found in Rhizoma coptidis ethyl acetate extract group,Rhizoma coptidis n-butanol extract group, Rhizoma coptidisis water extracts group andRhizoma coptidis decoction group compared with excess heat syndrome group.As compared to the blank control group, there were15items of significant genefunction in the excess heat syndrome group. There were34,31,53and29items ofsignificant gene function in Rhubarb ethyl acetate extract group, Rhubarb n-butanol extractgroup, Rhubarb water extracts group and Rhubarb decoction group respectively comparedwith excess heat syndrome group. There were59,78,72,32items of significant genefunction in Rhizoma coptidis ethyl acetate extract group, Rhizoma coptidis n-butanolextract group, Rhizoma coptidis water extracts group and Rhizoma coptidis decoctiongroup respectively compared with the excess heat syndrome group.Conclusion：Excess heat syndrome rats regulate body function mainly throughchanging the expression of genes related to metabolic processes, calcium ion homeostasisand xenobiotic stimuli response. Rhubarb decoction reduces the excessive energymetabolism of the body mainly through modulating the expression of genes involved inmetabolic processes, particularly glucose metabolism. Rhizoma coptidis Decoction plays aregulatory role in the rats with excess heat syndrome mainly through modulating theexpression of genes involved in metabolic processes, especially amino acid metabolism.The herbs with cold property such as rhubarb, Rhizoma coptidis and their extractsmay realize their antibacterial anti-inflammatory, antiviral, anti infection effect byincreasing the gene expression of Gclc, Adh1, Rpl6, Nqo1, RGD1562920_predicted anddecreasing the gene expression of Ubd, Hamp, Sds, LOC683385. The above genes may beone of the main molecular mechanisms of clearing away heat, purging fire and eliminatingtoxic materials of the Chinese medicinal herbs with cold nature.