Therapeutic Effects Of Ginkgo Biloba Extract In Animal Models Of Parkinson’s Disease

Parkinson’s disease(PD) is a chronic nervous system degenerative disease, in which the substantia nigra pars compacta dopamincrgic neuron selected lost and reduced the expression of dopamine in striatum. The Parkinson’s disease patient had a series of typical symptoms such as tremor, muscle stiffness and movement disorders. So far, no any kind of treatment can completely cure for PD. The traditional chinese medicine get more and more attention to treat PD. The main effective components of ginkgo biloba extract(GBE) were flavonoids and terpenoids lactone compound. The pharmacological studies shown that GBE has good protective effect on nerve, which may play an important role in the treatment of PD.Study the breed of α-syn A53 T transgenic mice and its genetic expression and physiological property. Study the pathogenesis of α-syn A53 T PD model and the pathogenesis of MPTP induced PD model. To investigate the therapeutical effect of Ginkgo Biloba extract(GBE) on different ages of PD mice that induced by MPTP and α-syn A53 T PD mice.1. Study the breed of α-syn A53 T transgenic mice and its genetic expression and physiological property.Twelve α-syn A53 T PD model mice were bought included nine female mice and three male rats. Feeding the mice 10 weeks after quarantine inspection passed and accouplement,which expected to produce 2 generations in next four month. Extraction the DNA from the mice tails of filial generation mice to amplify the α-syn A53 T gene by PCR and detect it by agarose gel electrophoresis. Blutabnahme to detect the blood routine examination.Executed the mice and Weigh the body wight and Each organ wight to calculate the organ coefficient. The results showed after α-syn A53 T gene identification, a total of 69 wild type mice and 79 α-syn A53 T transgenic mice. The blood routine examination had no obvious change in theα-syn A53 T transgenic mice compared with wild type mice. The organ coefficient had no obvious change in theα-syn A53 T transgenic mice compared with wild type mice except of splenic coefficient and kidney coefficient.2. Study the pathogenesis of α-syn A53 T PD model.Mice were randomly divided into two groups: wild type mice andα-syn A53 T PD model group which detect. The limb motor function of mice were tested by using pole-climbing, hanging and swimming test. The activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and the content of malondial dehyde(MDA) in the brain tissue were measure using spectro photography. The number of positive neurons expressing tyrosine hydroxylase(TH) in substantial nigra pars compacta and dopamine transporters(DAT) in striatum were detected by using the method of immunohistochemistry. The results showed the motor function score, the activity of GSH-Px, and the mean optical density of TH and DAT in brain tissues of model group of four month, six month and eight month significantly decreased compared with negative group, while the content of MDA was significantly raised, showing the model of PD was successfully established. The motor function score, the activity of SOD and GSH-Px, the expression of MDA, and the mean optical density of TH and DAT in brain tissues of α-syn A53 T PD model have significant change as α-syn A53 T PD mice grow older.3. Study the pathogenesis of MPTP induced PD model.C57BL/6 male mice were randomly divided into two groups: negative group and PD model group which were built through injecting with MPTP. The limb motor function of mice were tested by using pole-climbing, hanging and swimming test. The activities of SOD, GSH-Px and the content of MDA in the brain tissue were measure using spectro photography. The number of positive neurons expressing TH in substantial nigra pars compacta and DAT in striatum were detected by using the method of immunohistochemistry. The results showed The symptom such as body trembles, vertical hair, tail rigidity, gait instability, activity ability weakened were immediately turn up after injecting with MPTP. The motor function score, the activity of GSH-Px, and the mean optical density of TH and DAT in brain tissues of model group of two month and eight month significantly decreased compared with negative group, while the content of MDA was significantly raised, showing the model of PD was successfully established. The motor function score, the activity of SOD and GSH-Px, the expression of MDA, and the mean optical density of TH and DAT in brain tissues of two month old PD mice did not have significant change compared with eight month old PD mice.4. To investigate the therapeutical effect of Ginkgo Biloba extract(GBE) on different ages of PD mice that induced by MPTP and α-syn A53 T PD mice.Different ages of PD mice that induced by MPTP and α-syn A53 T PD mice wererandomly divided into five groups: model group, positive group, high, medium and low dose GBE group, respectively. The limb motor function of mice were tested by using pole-climbing, hanging and swimming test. The activities of SOD, GSH-Px and the content of MDA in the brain tissue were measure using spectro photography. The number of positive neurons expressing TH in substantial nigra pars compacta and DAT in striatum were detected by using the method of immunohistochemistry. The results showed the function score, the activities of SOD and GSH-Px, the mean optical density of TH and DAT in brain tissues of the GBE treatment group significantly raised compared with the PD mice that induced by MPTP, while the content of MDA was significantly decreased, the treatment effect in the two month PD mice had no significant change compared with eight month PD mice.(2) The function score, the activities of SOD and GSH-Px, the mean optical density of TH and DAT in brain tissues of the GBE treatment group significantly raised compared with theα-syn A53 T PD mice, while the content of MDA was significantly decreased.The results showed A total of 69 wild type mice and 79 α-syn A53 T transgenic mice after breeded to apply to subsequent experiments. The blood routine examination and organ coefficient had no obvious change in theα-syn A53 T transgenic mice compared with wild type mice. The motor function score, oxidation resistance, and the mean optical density of TH and DAT in brain tissues have significant fall off as α-syn A53 T PD mice grow older.The motor function score, oxidation resistance, and the mean optical density of TH and DAT in brain tissues have significant fall off after injecting with MPTP. The preventive medication of GBE shows a protective effect on PD mice.