Differentially Expressed In Psoriatic Peripheral Circulation Of Blood MiRNA And The Intervention Effects Of ZhuHuang Granule

Psoriasis is difficult to cure human common chronic inflammatory skin disease. Psoriasis patients throughout the world, of different ethnic, gender, age may be the disease, spring and winter recur or worsen, its incidence was significantly increased in recent years the trend. The pathogenesis of the disease is very complex genetic epidemiology and genetics studies have confirmed that it is a multifactorial inheritance pattern of complex diseases involving the interaction of genes and environment interactions and multiple genes. With the gradual deepening of domestic and international academic research in the field of small RNA molecules, found in a variety of miRNA play an extremely important role in skin physiology and pathology process. We also found by previous studies of miRNAs in psoriatic lesions, mononuclear cells express specific differences are present. In recent years, patients with normal plasma miRNAs with different diseases to detect differentially expressed miRNAs found, and as physiology, disease type and length of the course change, which confirmed that the blood circulating miRNA is a potential diagnostic marker thereof. Therefore, from the genetic level of early diagnosis of psoriasis, treatment and prevention and other topics in the future is bound to become one of the industry’s major research directions.Purpose:Use file retrieval, determined10with psoriasis pathogenesis-related miRNAs, and then go on plasma miRNA screening differentially expressed by RT-qPCR technology, miRNA expression bioinformatics analysis provides further clues for the latter difference, also provide ideas for early diagnosis and treatment of psoriasis. At the same time observe the effect of plasma treatment Zhuhuang particles differentially expressed in patients with psoriasis vulgaris miRNA expression changes of serum cytokines and explore traditional Chinese medicine treatment of psoriasis vulgaris mechanism of action, provide an objective basis for the prevention and treatment of psoriasis TCM.Methods:The first part called10psoriasis patients and10healthy subjects, the first collected five cases and five cases of patients with normal physical examination of peripheral blood plasma samples. And extracted with Trizo method RNA, detect the total, then experimental verification miRNA expression differences10normal human plasma in patients with psoriasis by miRNA RealTime RT-PCR, to determine the five fold difference greater than4miRNA, and then in the remaining specimens with the same method to authenticate again.The second part called psoriasis vulgaris48volunteers two months Zhuhuang granules treatment. Before calculating the difference of treatment five miRNA and related inflammatory cytokines and PASI integral. And changes in miRNA expression analysis before and after treatment, while using the differences in serum concentrations of cytokines by ELISA before and after treatment.Results:1.miRNA differentially expressed in peripheral blood plasma in patients with psoriasis, wherein the5-specific miRNA (miR-146a, miR-155, miR-210, miR-142-3p, miR-18la), respectively, showing a decrease in up-regulated trend.2.miR-146a, miR-155, miR-210is positively correlated with PASI integration; miR-142-3p, miR-181a negatively correlated with PASI integration. In addition to the yard because of inflammatory IL-4, IL-10showed a negative correlation, and the rest showed a positive correlation.3.Zhuhuang particle treatment of psoriasis, the total effective rate70.83%, and for five miRNA and differential expression of inflammatory cytokines in the regulation can.Conclusion:1.miRNA differentially expressed in the presence of psoriatic peripheral circulation in plasma, we validated by RT-qPCR miR-146a and other five differences miRNA;2. Five miRNAs differential expression of inflammatory cytokines and PASI integral exhibit positive or negative correlation;3.Chinese medicine treatment of psoriasis Zhuhuang granules significant effect;4.Chinese Zhuhuang Granules on four specific miRNA regulation have significant effect on the miR-181a no significant changes; inflammatory cytokines in addition to IL-10were outside the rest of regulation.5.For the next thing to carry out the functions and molecular markers miRNA target genes provides an experimental basis.