Analysis Of Screening Differential Genes Of Coronary Artery Disease In The Uygur, Kazakh, And Han Population Of Xinjiang

Objective:1) To identify differential genes of coronary artery disease in the Uygur,Kazakh, and Han population of Xinjiang using gene chip expression profiles.2) Toexplore the associations of genetic polymorphisms of Early growth response3(Egr3) andcoronary coronary artery disease in the Uygur and Han of Xinjiang.3) to investigate therelationship between genetic polymorphisms of PAI-2and prognosis of coronary arterydisease in the Uygur and Han of Xinjiang. Methods:1) A case-control study including9cases (3Uygurs,3Kazakhs, and3Hans) with coronary heart disease confirmed bycoronary angiography and9gender-, age-, and ethnic-matched normal controls wasdesighed for the present study. In this study global expression patterns of were analyzedby Affymetrix Human Geonme U133+2.0chip was employed to find out differentialgenes expression about coronary artery disease.2) A case-control study including709cases with coronary heart disease confirmed by coronary angiography and654gender-,age-, and ethnic-matched controls was desighed to analyse the relationship between Egr3SNPs (rs1996147and rs1008949) and CAD. Genotyping was undertaken using theTaqMan SNP genotyping assay.3) A case-control study was designed to observe therelationship between genetic polymorphisms of PAI-2and prognosis of coronary arterydieases.674patients with coronary heart disease and816control subjects weredetectived by the TaqMan SNP genotyping assay. All these374patients were fellow-upfor one year, we observed the relationship between different genotype and prognosis ofcoronary heart disease. Results:1)33genes were differentially expressed associatedwith coronary heart disease.43genes were differentially expressed between Uygurpatients with coronary heart disease and Han patients with coronary heart disease.2)rs1996147in Egr3gene was associated with coronary artery disease in Uygur ethnic inXinjiang. Rs1996147AA genotype significantly increased the risk of coronary heart disease, after adjustment for other factors, rs1996147gene polymorphism was stillindependent risk factors for coronary heart disease; Rs1996147gene polymorphismsignificantly affected the severity and extent of CAD coronary arterial stenosis.3)Rs8093048in PAI-2gene was associated with coronary artery disease in both Han ethnicand Uygur ethnic in Xinjiang. Rs8093048TT genotype significantly increased the risk ofcoronary heart disease in Han and decreased the risk of coronary heart disease in Uygur.After one year follow-up, by COX multivariate regression analysis showed that afteradjusting for other factors the rs8093048TT polymorphism in PAI-2is an independentrisk factor of cardiovascular adverse events in coronary heart disease of Han. There wasnot association of rs8093048TT polymorphism with adverse prognosis of coronary heartin Uygur. Conclusion:1) This study of coronary artery disease in the Uygur, Kazakh,and Han population of Xinjiang by gene chip expression profiles showed that both theinnate and adaptive immune systems contributed to the pathogenesis of coronary arterydisease. Expression of Egr3and PAI-2genes were different between Uygur and Hanpatients with coronary artery disease.2) Rs1996147gene polymorphism of Egr3wasassociated with coronary heart disease in Uygur ethnic, and significantly affected theseverity and extent of CAD coronary arterial stenosis.3) Rs8093048gene polymorphismof PAI-2was associated with coronary artery disease in both Han ethnic and Uygurethnic in Xinjiang. Rs8093048TT genotype is an independent risk factor ofcardiovascular adverse events in coronary heart disease of Han.