Study On Effects Of Acupuncturing Points In Behavior Change Of Mania Rats And Its Action Mechanism

Background:we observed behavior change of mania rats treated by acupuncture points and changes of part of the central neurotransmitter’s content. We hope to find objective evidence from macroscopic and microcosmic.Objective:We researched the specificity of acupoints through observing changes of body weight, food intake, the incubation period of sleep of mania rats treated by acupuncture, non-points and valproate. We also discussed the mechanism of acupuncture’effect on mania, through detecting the content of DA and5-TH in the peripheral blood, GABA and GLU neurons in the hippocampus and EAAT3gene expression.Materials and methods:We chose50SD rats, half male and half female, which were divided randomly into four groups after feeding1week, with10rats in the control group, model group, acupuncture group, non-point group and valproate group. The modified multiple platform method (MMPM) was used to establish mania rats model. We did nothing to the rats in the control group and model group, but do acupuncture therapy to the rats in the acupuncture group and non-point group.30minutes each time the needle,1times a day. The rats in the valproate group were given suspension of valproate by intragastric administration (300mg/kg),1times a day. After7days, we observed changes of body weight, food intake, the incubation period of sleep of mania ratsand and open field test. The eighth day, the rats were killed, then we detected the content of neurotransmitter dopamine in the peripheral blood, GABA and GLU neurons in the hippocampus and gene expression.Results: 1. After the treatment, the body weight, food intake and activity emotion of acupuncture group and valproate group were compared with model groups, they were statistically significant, which indicated that acupunctures and valproate had effects of increasing body weight, food intake, and easing the activity emotion. The body weight, food intake and activity emotion of non-point group with that of the model group, they was not statistically significant (P>0.05). The incubation period of sleep of acupuncture group, valproate group and non-point group were compared with the model groups, they were statistically significant, and the acupuncture groups were compared with valproate group, they were statistically significant, which indicated that acupuncture points had effective specificity.2. The level of Glu and GABA of acupuncture group and valproate group were compared with the model group, they were statistically significant.3. The level of DA and5-TH of acupuncture group and valproate group were compared with the model group, they were statistically significant.4. The expression of EAAT3in acupuncture point group was higher than that in valproate group, and non-point group’effect was similar. The acupuncture group can see clear Protein Electrophoresis Band.Conclusions:1. Acupuncture points and valproate had effect of increasing body weight, food intake, and easing the activity emotion. Among the three methods, the acupuncture points have curative effect, valproate’s effect took the second place, non-point group’effect was similar.2. The level of Glu decreased and that of GABA increased in the acupuncture points group and valproate group, non-point group’effect was similar.3. The level of DA decreased and that of5-TH increased in the acupuncture points group and valproate group.4. Acupuncture point’s effect of improving the manic mood was related to reinforced expression of EAAT3in Hippcampus of rats.