| Background:In the world, type2diabetes is the third disease, following the cancer and thecardiovascular diseases. It is harm to human health seriously..Type2diabetes is a complexdisease, is the result of multiple risk factors, including environmental factors and geneticfactors. With the improvement of people’s living standard and the diversification of life,type2diabetes prevalence is increasing year by year.Participate in the pathogenesis of type2diabetes mellitus risk factors there are many, such as age, gender, race, family history,obesity, hypertension, etc.. These risk factors interact with one another, its mechanism isvery complicated.The risk factors of type2diabetes have a large number of literaturereports, including the change of factors and can change the factor two. The change offactors include genetic factors and genetic effect. As everyone knows is type2diabetesmellitus with distinct genetic tendency; genetic factors mainly include such as: familyhistory, genetic and ethnic and regional differences in three aspects.This will be part of acase-control study to explore the Chongqing area influence the occurrence of type2diabetes environmental risk factors, expectations for the positive effective diabetesprevention and control work to provide the experimental basis for further study of theenvironment, at the same time a gene that may exist between the interaction to do somefoundation workObjective:A case-control study was disigned to investigate the environmental risk factors of type2diabetes. Method:The type2diabetes mellitus (T2DM group) were enrolled500patients, average age(55.4±12.2) years. Male349, female151. The control group were enrolled500cases, theaverage age (55.6±11.7) years; male332cases, female168cases. Multivariateunconditional logistic regression analysis to screen risk factors for type2diabetes.Results:The single non-conditional logistic regression analysis showed that, in selectedvariables, and the incidence of type2diabetes was significantly positively correlatedvariables: a history of smoking, drinking history, life stress, family history of CAD, familyhistory of diabetes, eight risk factors such as obesity; Multivariate Logistic regressionanalysis showed that independent risk physicians selected for the main effects model as ahistory of smoking, life stress, family history of diabetes, and obesity.Conclusion:Type2diabetes and smoking, obesity, life stress, family history of diabetes exist inclose contact, but also between the various factors are interrelated. Therefore, early andtimely of these risk factors, a comprehensive intervention to control blood pressure, bloodlipids, weight, healthy lifestyle, not smoking, not drinking, appropriate to participate insports, there is important to control the occurrence and development of type2diabetes andimpact. BackgroundWith economic and society development, incidence of type2diabetes increased yearby year.From developed countries to developing countries,type2has played an importantrole causing vascular diseases especially coronary heart disease. Recent decades studiessuggest that type2diabetes is a complex multifactorial disease, in addition toenvironmental factors including diet and lifestyle changes in the factors, genetic variabilityin patients with type2diabetes important risk factors. In the past decade, has been througha lot of effort to find the type2diabetes susceptibility gene, but progress has been slowerthan expected. Greatly increased interest in recent emerging genome-wide associationstudies (GWASs) method so that people looking for a type2diabetes susceptibility genes,proved also to accelerate the discovery of a type2diabetes susceptibility gene.41.62kb,SLC30A8gene is located in8q24.11, zinc transporter protein8(ZnT-8) encoding geneSLC30A8gene-specific expression in pancreatic B cells. The main role of ZnT-8as one ofthe members of the zinc transporter family, only expression in pancreatic β-cells, and theplay must mature insulin and/or storage of zinc ion in the pancreatic beta cells to secreteinsulin. ZnT-8is a protein composed of369amino acid residues. Studies have shown thatnon-synonymous mutations in the SLC30A8gene in exon Arg325Trp association is afunction of polymorphic loci, and the occurrence of type2diabetes. Some studies suggestthat there is a link SLC30A8polymorphisms and β-cell dysfunction. Despite the associationbetween these studies found that the SLC30A8gene polymorphism and type2diabetes, butmost of the studies have focused on the SNP single locus haplotype, which rarely build.Objective: In recent genome-wide association studies, variants in SLC30A8genewere associated with risk for type2diabetes. In the present study, we have established thehaplotypes of the SLC30A8gene three SNPs (rs2466295, rs4876703, rs11558471), and toassess the association between these haplotypes and type2diabetes.The aim of this study was to assess the Association of tag SNPs spanning SLC30A8and their haplotypes withtype2diabetes in Chinese Han population.Methods:1. Type2diabetes mellitus (T2DM group) were enrolled in the1508cases, averageage (53.4±12.3) years. All subjects were selected from January2009to October2011,Department of Endocrinology, Southwest Hospital, Third Military Medical University inhospitalized patients, in line with the1999World Health Organization (WHO) diagnosticcriteria for type2diabetes. Exclude type1diabetes, mitochondrial diabetes, gestationaldiabetes, malignancy.2. Control group were enrolled in the1500cases, the average age (53.6±11.8) years;the control choice from healthy people healthy in our hospital, underwent fasting glucoseand OGTT experiments exclude diabetes3. The population of cases and controls were collected demographic data, and general,personal history and family history, smoking and drinking history, past history, medicationhistory, and measurement of blood pressure, height, weight, body mass index (BMI), andother indicators. Control the option of following the age, sex, ethnic group and T2DMgroup match. Before the groups included in the study signed an informed consent.4. There were1508Chinese Han type2diabetes patients and1500age-andsex-matched control subjects genotyped for3tagging SNPs (rs2466295, rs4876703, andrs11558471) of the human SLC30A8gene.5. Haplotypes were constructed and their frequencies compared between the type2diabetes patients and the controls.Results:1．Comparison between the control group and the patient group, age, uric acid,triglycerides, total cholesterol, alcohol consumption, pulse was no significant difference2．We obtained three of the SLC30A8gene tag SNPs (tagging SNP), respectively,rs2466295, rs4876703, and rs115584713tags SNP is located in the same monomer typeregion (Figure1A and1B represent a region of the SLC30A8gene23kb,), also on behalf ofthe other14SNP information.3．Of the SLC30A8gene SNP genotype distribution are in line with the Hardy- Weinberg equilibrium (P>0.05), with group representation.4．The AA genotype of rs11558471was found more frequently in the type2diabetespatients than in the control subjects (46%vs24%; P <0.001). The frequencies of the A-C-A haplotype was significantly higher in the type2diabetes patients than in the controlsubjects (0.331vs0.120; P<0.001). The frequency of the A-C-Ghaplotype was markedlylower in the type2diabetes patients than in the control subjects (0.160vs0.365; P<0.001).Conclusions:The present results indicate that type2diabetes is associated with the AA genotype ofrs11558471in the human SLC30A8gene. The A-C-A haplotype appears to be risk factorsand the A-C-G haplotype may be a protective factor of type2diabetes in Chinese Hanpeople. |
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