Chronic Prostatitis And Proctitis Correlation Of Experiment Research And Xiong Zhen Cream For Evaluation Of The Intervention

Purpose:The objective of this study was to explore the correlation between chronic prostatitis and proctitis, and its possible mechanism. Authors observed the therapeutic effect and improvement of behavior of rat models with chronic prostatitis and proctitis by external application of traditional Chinese medicine Xiongzhen ointment medication, so as to extend clinical prospect for chronic prostatitis.Methods:authors used model drugs with three different concentrations to replicate rat models with chronic prostatitis, and set sham operation control group. After a week’s modeling, authors divided rat models with different concentration drugs into medication group and non-medication group randomly. The medication group was treated by daily retention enema with Xiongzhen ointment, while non-medication group was fed with normal diet. After three weeks’treatment, authors observed the inflammation-related changes in prostate and rectum of each rat in the following methods:observation of pathological structure changes by hematoxylin-eosin staining, detection of the expression of IL-17and COX-2by immunohistochemical method, determination of the expression of IFN-r and TNF-a by ELISA method, and research of the expression of NGF and TRPVlmRNA by RT-PCR method in spinal cord cell in lumbosacral region, which were in common innervation system. In non-medication group, authors could observe whether the prostate and rectum of rats with different concentration drugs has the relevant of inflammation expression and its possible production mechanism, while rats with same concentration drug could compare the therapeutic effect of Xiongzhen ointment between non-medication group and medication group. In the process of rats feeding after the operation, authors could observe the difference in behavior change of rats in each group at the same time.Results:1. Model rats in different concentration drugs with chronic prostatitis could initiate proctitis, and those with high concentration drug had the most obvious effect.2. Among model rats with high concentration drug, the pathological structure changes in prostate and rectum tissues, the expression of inflammatory mediators IL-17, COX-2, IFN-r and TNF-α in medication group was obviously lower than that in non-medication group, but there was no significant difference in model rats with medium and low concentration drugs.3. Among model rats with high, medium and low concentration drugs, either medication group or non-medication group, the expression of NGF and TRPVlmRNA in spinal cord cell in lumbosacral region had no obvious difference.4. Among model rats with high concentration drug, the behavior change in medication group was better than that in non-medication group, but there was no significant difference in model rats with medium and low concentration drugs.Conclusions:there was a correlation between prostatitis and proctitis indeed, but this experiment could not yet conclude that they had dose-effect relationship. Xiongzhen ointment was effective to both diseases, and it could reduce rats’bad behavior and improve the quality of experimental animals’lives in the meantime. This correlation may be related with cross-organ sensitization, and it was valuable in the clinical treatment of chronic prostatitis when treating proctitis at the same time.