Single-Cell Multiomics Analysis Presents The Landscape Of Peripheral Blood T-Cell Subsets In Human Chronic Prostatitis/Chronic Pelvic Pain Syndrome

Background: Chronic prostatitis is characterized by feeling pain in the structure related to the pelvis for at least six months,but no infection or other obvious local pathology is confirmed.In the outpatient department of urology,about 25% of patients with chronic prostatitis,and about 50% of men have experienced prostatitis-like symptoms to varying degrees in their lifetime.Although multimodal therapies seem to be the most successful,CPPS remains a major challenge because available treatments often fail and there is still a lack of "panacea",which is also a cause of frustration for patients and doctors.At present,one of the most pressing problems in the study of the pathogenesis of prostatitis is the lack of a recognized model.Evidence shows that abnormal T lymphocyte differentiation affects the pathogenesis of chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS).Therefore,understanding the immune activation of CP/CPPS will help improve treatment strategies.Method: First,Ficoll gradient centrifugation of human peripheral blood samples was used to isolate peripheral blood mononuclear cells,and BD?Ab Seq was used to analyze the protein and gene expression levels of single peripheral T cells from two CP/CPPS patients and two healthy controls.Quantification of numbers.We used a comprehensive strategy,based on a typical correlation analysis of more than 10,000 Ab Seq profiles,that is,using the BD Rhapsody? T cell panel and the BD?Ab Seq test to draw a T cell immune composition map to identify CP/CPPS-related functional T cells Subgroup.Finally,the preliminary verification was carried out by flow cytometry.Results: 15 unique T cell subgroups were identified.It is worth noting that we found that the proportion of cluster 0(30.35%)in the CP/CPPS group was significantly higher than that of the healthy control group(9.38%);cluster 0 was defined as effector T cells based on differentially expressed genes/proteins.Flow cytometry test confirmed that the effector T cell subsets in the peripheral blood mononuclear cells of CP/CPPS patients,especially the central memory T cells,helper T cells(Th)1,Th17 and Th22 cells were significantly higher than The healthy control group(P<0.05)further confirmed that the abnormality of effector T cells may cause or strengthen CP/CPPS.Conclusion: Our findings describe the complex arrangement of T cell states in human blood,and suggest that effector T cells,especially central memory T cells,T helper T cells(Th)1,Th17 and Th22 cells,and Treg cells,It may be the cause of CP/CPPS.