Study Of Treatment And Mechanisms Of SGHWJN Particles On Esophagitis In Rats

Part one Treatment effects of SGHWJN particles on rat with reflux esophagitisObjective:To study the influence of SGHWJN particles on gastroesophageal pH value, Pepsin, esophageal mucosal histomorphology and expression of PCNA in esophageal tissue of rats with reflux esophagitis. Methods:Fifty SD rats were randomly divided into 5 groups, namely, a control group, a sham-operated group, a model group, a SGHWJN particles group and a PPI group. Reflux esophagitis was induced by adopting partial pyloric ligation plus cardiomyotomy. One week later, the rats were orally administered twice daily for 28 days. Pathological changes and Cell ultrastructure of esophagus mucous membrane were evaluated by using HE staining and electron microscope respectively every groups. Gastroesophageal pH and Pepsin were determined and expressiones of PCNA in esophageal tissues were examined by Western-blotting. Results: Gastroesophageal pH value of the model group was significantly lower than that of control group and sham-operated group(P<0.05);Pepsin, esophageal mucosal hyperplasia, Intercellular Space and expression of PCNA mRNA in esophageal tissues of model group, compared with the normal group and sham operation group, were significantly higher (P<0.05);Gastroesophageal pH value of SGHWJN particles group and PPI group were significantly higher than that of model group (P <0.05); Pepsin, esophageal mucosal hyperplasia. Intercellular Space and expression of PCNA mRNA in esophageal tissues of SGHWJN particles group and PPI group than those in model group decreased significantly (P<0.05), no statistically differences on the above-mentioned indicators between SGHWJN particles group and PPI group(P> 0.05). There were no statistically differences on the above-mentioned indicators between the normal group and sham-operated group (P> 0.05). Gastroesophageal pH value were negatively correlated with histological scores (r=-0.793). Pepsin, esophageal mucosal hyperplasia, Intercellular Space and expression of PCNA mRNA in esophageal tissue and histological scores were positively correlated (r=0.853). Conclusion:SGHWJN particles can effectively regulate Gastroesophageal pH value, Pepsin, esophageal mucosal hyperplasia, Ultrastructure and inflammation in esophageal tissues in rat with reflux esophagitis.Part two Influence of SGHWJN particles on serum GAS, VIP, SP, NOS, SOD and MDA in rat with reflux esophagitisObjective:To study the influence of SGHWJN particles on serum GAS, VIP, SP, NOS, SOD and MDA in rat with reflux esophagitis. Methods:Fifty SD rats were randomly divided into 5 groups, namely, a control group, a sham-operated group, a model group, a SGHWJN particles group and a PPI group. Reflux esophagitis was induced by adopting partial pyloric ligation plus cardiomyotomy. One week later, the rats were orally administered twice daily for 28 days. After treatment, serum GAS, VIP, SP, NOS, SOD and MDA were determined. Results:Serum NOS and MDA of the model group was significantly higher than that of control group and sham-operated group(P<0.05); Serum SP, VIP, GAS and SOD of model group, compared with the normal group and sham operation group, were significantly lower (P<0.05); Serum NOS and MDA of SGHWJN particles group and PPI group were significantly decreased than that of model group (P <0.05); Serum SP, VIP, GAS and SOD of SGHWJN particles group and PPI group were significantly higher than those in model group (P<0.05), SGHWJN particles group and PPI group, no statistically differences between the above-mentioned indicators (P> 0.05). There were no statistically differences on the above-mentioned indicators between the normal group and sham-operated group (P> 0.05). Histological scores and Serum NOS and MDA were positively correlated (r=0.813). Serum SP, VIP, GAS and SOD were negatively correlated with histological scores (r=-0.847).Conclusion:SGHWJN particles can effectively inhibit Serum NOS and MDA and induce Serum SP, VIP, GAS and SOD in rat with reflux esophagitis. Part third Study of expression of FOXO4 signaling pathway in esophageal tissue of rat with reflux esophagitisObjective:To study the expression of FOXO4 signaling pathway in esophageal tissue of rat with reflux esophagitis. Methods:Thirty SD rats were randomly divided into 3 groups, namely, a control group, a sham-operated group and a model group, each group 10 rats. Reflux esophagitis was induced by adopting partial pyloric ligation plus cardiomyotomy. Four weeks later, the rats were sacrificed. Pathological changes of esophagus mucous membrane were evaluated by using HE staining and the expression of PI3K/AKT/FOXO4/NF-KB/TNF-a mRNA in esophageal tissues were examined by real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) with SYBR Green. Results:Compared with the normal group and sham operation group, esophageal histological scores and expression of PI3K, AKT, NF-κB and TNF-a mRNA in esophageal tissues of model group were significantly higher (P<0.05), expression of FOXO4 mRNA were significantly decreased (P<0.05). Expression of P13K, AKT, NF-κB and TNF-a mRNA in esophageal tissue and histological scores were positively correlated (r=0.872). Expression of FOXO4 were negatively correlated with histological scores (r=-0.831). Conclusion: The changes of expression of FOXO4 signaling pathway in esophageal tissue take part in the genesis and development of reflux esophagitis.Part fourth Effects of SGHWJN particles on expression of FOXO4 signaling pathway in esophageal tissue of rat with reflux esophagitisObjective:To study the effects of SGHWJN particles on the expression of FOXO4 signaling pathway in esophageal tissue of rat with reflux esophagitis and to explore the possible mechanism. Methods:Thirty SD rats were randomly divided into 3 groups, namely, a model group, a SGHWJN particles group and a PPI group. Reflux esophagitis was induced by adopting partial pyloric ligation plus cardiomyotomy. One week later, the rats were orally administered twice daily for 28 days.The expression of PI3K/AKT/FOXO4/NF-KB/TNF-a mRNA in esophageal tissues were examined by real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) with SYBR Green. Results:Compared with the model group, expression of PI3K, AKT, NF-κB and TNF-αmRNA in esophageal tissues of SGHWJN particles group and PPI group were significantly decreased (P<0.05), expression of FOXO4 mRNA were significantly higher (P<0.05); There was no significant difference between SGHWJN particles group and PPI group (P>0.05). Expression of PI3K, AKT, NF-κB and TNF-αmRNA in esophageal tissue and histological scores were positively correlated(r=0.872). Expression of FOXO4 were negatively correlated with histological scores (r=-0.831). Conclusion:SGHWJN particles can effectively inhibit pathological injury in rat with reflux esophagitis through regulating expression of FOXO4/NF-KB/TNF-αsignaling pathway.