Toxic Effects And Detection Technology Of Benzo[a]Pyrene On Clam Ruditapes Philippinarum

According to the situation of China’s offshore PAHs pollution, the auther studied the metabolic mechanism of Ruditapes philippinarum exposed to B[a]P, PBTK-TD model in clam exposed to B[a]P and marine PAHs pollution monitoring technology based on Ruditapes philippinarum. This study identified detoxification enzyme activity, damage effect, the related gene expression of Ruditapes philippinarum under the stress of B[a]P and the metabolic mechanism of Ruditapes philippinarum exposed to B[a]P. Moreover, this study comprehensively selected the effective biomarkers, developed the PBTK-TD and demonstrated the effectiveness of biomarkers through field sampling experiments.1Study on metabolic mechanism of Ruditapes philippinarum exposed to B[a]PThis paper studied the metabolic pathway of Ruditapes philippinarum exposed to B[a]P, and clarified cumulative distribution of B[a]P and its metabolites in Ruditapes philippinarum. When B[a]P entered the clam, a variety of phase Ⅰ metabolic enzymes were activated, resulting in a large number of phase Ⅰ metabolites. Moreover, due to inhibition of GST and induction level of UGT and SULT was relatively low, a large number of B[a]P phase Ⅰ metabolites accumulated in tissues of clams. Subsequently, the phase Ⅱ metabolism enzyme substrate (phase Ⅰ metabolites) increased, the speed of B[a]P phase Ⅰ metabolites into phase Ⅱ metabolites gradually increased, eventually leading to the generation rate and metabolic rate of B[a]P phase Ⅰ metabolites reached balance. Similar to B[a]P phase Ⅰ metabolites, the discharge rate of B [a] P Ⅱ phase metabolites gradually approaching its formed speed, leading to the level of B[a]P phase Ⅱ metabolites tends to be stable, no significant differences in metabolic enzymes and the cumulative effect of the Philippines clam gill and digestive gland.2Study on PBTK-TD in Ruditapes philippinarum exposed to B[a]PThrough correlation analysis, the auther selected out the enzyme activity, damage effect, gene expression and other aspects of the biological markers, such as AHH, GST, SOD, DNA alkaline unwinding and AhR, GST-Pi, Mn-SOD mRNA gene expression, as well as developed the; PBTK-TD, linking the process of B[a]P absorption, distribution, metabolism, excretion of Ruditapes philippinarum and biological effect.3Study on marine PAHs monitoring technology based on Ruditapes philippinarum The results in Qingdao offshore showed that PAHs pollution in Yinghai was the most serious, followed by Hongdao, Fengcheng and Jiaonan. The results in Laizhou Bay showed that PAHs pollution in Guangligang was the most serious, followed by Xiaying and Nanhaipu. PAHs concentrations in December were the highest, but season hardly affected PAHs accumulation and biomarkers in Ruditapes philippinarum.According to the correlation analysis, AHH activity and mRNA expression of AhR gene had good correlation with PAHs accumulation in Ruditapes philippinarum. As a result, they can be used as useful biomarkers in marine PAHs monitoring.