| Percutaneous implant is implanted through the skin and keep the skin open. It is mainly used as retainer in the maxillofacial defects and limbs defects. Untill now,there is no proper materials that could be applied to percutaneous implant in clinics. If it is not achieved the seal between the implant and the skin, it must lead to infection of implants and epithelial migration, ultimately the implant fails. To achieve percutaneous seal, in addition to its proper shape must be designed optimally to reduce the stress concentration and the relative movement, it must ensure close attachment of percutaneous implant surface and different tissues of the body, as well as have some anti-infection abilities.In this study, titanium as an implant material, some halogen ions would be added into electrolyte and the properties and the biological behavior of oxide layer were prepared by micro-arc oxidation technology. The anti-bacterial efficiency,biocompatibility, effect on a human skin epidermal cell adhesion and proliferation, biological activity in a simulated body fluid and the anti-corrosion abilities of the oxide layer prepared by micro arc oxidation would be evaluated. Finally, after the preparation of the implant surface treatment, the implant was implanted into the body of the animals in vivo tests. The results are as follows: 1. The physical, chemical and biological properties of the oxide layer by MAO treatment. The composition of the oxide layer can be changed after adding halogen compounds in the electrolyte, the layer would contain halogen ions.By anti-bacterial experiments and bacterial adhesion test, the oxide layer had antibacterial functions to common skin bacteria . The anti-bacterial properties MAO250 group was better.2. L-929 cells in vitro cytotoxicity of titanium layer containing halogen ions prepared by micro-arc oxidation was evaluated by MTT method and the oral mucosa irritation test was also carried out. The results showed that: the oxide layer containing halogen ions were non-toxic to fibroblast and non-irritating to the oral mucosa.3. The surface of titanium by micro-arc oxidation treatment was beneficial to early attachment and proliferation of epidermal cell. The surface of MAO250 group had better cell compatibility to epidermal cells.4. The biological activity of samples in each group were evaluated by the simulated body fluid immersion test. After immersing, different voltages, different treatment methods and different immersing time, concentration of calcium ions are different. In this experiment, MAO250 group oxide layer contained high content of calcium ions, and showed a good biological activity.5. Micro-arc oxidation surface treatment techniques could effectively improve anti-corrosion of the titanium surface to the sweat . The the anti corrosion capability of MAO400 group was better.6. After micro-arc oxidation treatment, there was excellent tissue compatibility of titanium, its proper aperture and surface structure could lead to the tissue growing into materials, and could effectively improve the binding force between the soft tissue and the material, the bonding strength of MAO400 group was better.7. By adding halide ions, titanium implant treated by the micro-arc oxidation was not only had a good bonding with soft tissue, but also could be tightly bonding with bone with the form of bone linkage with good performance and biocompatibility, it played a very important role in percutaneous sealing formation. |
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