A Metabonomics Study Of Acupuncture For Migraine With Acupoints Along Shaoyang Meridian

Objective:Used the nuclear magnetic resonance (NMR) based metabonomic technology to determine the metabolic profiles in the plasma samples of acute migraine rat models and compare the plasma ~1H-NMR metabolic profiles in different acupuncture groups. Used the NMR-based metabonomic technology to determine the metabolic profiles in the migraineurs'plasma samples and observe the changes in plasma ~1H-NMR metabolic profiles after acupuncture therapy. To explore the biologic features of specific points belong Shaoyang Meridians for migraine therapy.Methods:1. 50 female SD rats were randomly administrated to five groups:normal control group,sodium injection control group,acute migraine model group, acupuncture group1 (electro-acupuncture therapy with special points belong to Shaoyang Meridian) and acupuncture group 2(electro-acupuncture therapy with none-points along Shaoyang Meridian). The acute migraine model rats were induce by Nitroglycerin (NTG) injection according to the CRISTINA report. Plasma sample was collected after acupuncture treatment and determeined by ~1H-NMR, he metabolic profiles were analyzed by pattern recognition technology.The plasma'H-NMR metabolic profiles were compared between different groups.2. Including 9 female migraineur without aura, seleced special points belong to Shaoyang Meridian for acupuncture treatments, plasma sample was collected before and after acupuncture treatment and determined by ~1H-NMR, the metabolic profiles were analyzed by pattern recognition technology. The plasma ~1H-NMR metabolic profiles before and after acupuncture treatment had been compared to plasma~1H-NMR metabolic profiles of healthy female.Result:1.There are special metabonomics spectras of plasma in acute migraine rats models. The significant different in the plasma ~1H-NMR metabolic profiles resulted in increase in Lac(lactic acid),bata hydroxybutyric acid (3-HB),Glucose,glutamic acid/glutamine (Glu/Gin), creatinine(Cre) level and decrease in lipid, VLDL/LDL, Choline (Cho), N-acetyl-glycoprotein (Nac)2. The plasma'H-NMR metabolic profiles in sodium injection control group was less abnormal compred to nomal control group, and had obvious difference to NTG injection rats'; 3. The plasma ~1H-NMR metabolic profiles in different acute migraine rats groups indicated that the Lac, Glucose,Glu/Gln content were decreased in acupuncture group 1; the Lac content in in acupuncture group 2 also decreased, but the Glucose,Glu/Gln content had no obvious change in acupuncture group2;4. The plasma ~1H-NMR metabolic profiles of migraineurs without aura were compared to healthy female'.There were significant decrease in the contents of Lac,Glucose,Nac and increase in Glu/Gln,alanine (Ala),unsaturated fatty acid (UFA),Cho,VLDL/LDL in migraineurs'plasma;5. The acupucnture therapy had effects on adjusting the variation on Glu/Gln,Nac,Oac,Ala in migrainures'plasma to normal control levle, but had few effects on Lac, Glucose and Lipid level.Conclusion:1. The NTG injection might cause the blood vessel dilatation and induce the pain and neurogenic inflammation and caused the plasma ~1H-NMR metabolic profiles change.The acupuncture group1 were superior than acupuncture group2 in adjusting the micromolecular substance(Glucose,glutamic acid) content in migraine rats' plasma.Thespecial effects of special points belong to Shaoyang Meridian for migraine might related to the adjusting effects on Glucose,glutamic acid).2. The abnormal high level of glutamic acid might related to the peripheral algesia sensitization and disfunction of platelet;specific points along Shaoyang Merdian could regulate the variation on micromolecular substance content in migrainures' plasma. The Glucose and Lipid results in migrainures'plasma should be confirmed in the future studies.