Study On Regulating Function Of Chang Yu Ning Granula On Cytokine Network Factors Of Rats Models With Ulcerative Colitis

Objective:Discussing the basic pathogenesis of ulcerative colitis andinvestigate the regulating effects on cytokine network factorsTLR4,NF-kB,TNF-a of active ulcerative colitis in rats,with Chang Yu NingGranula,a experiential decoction which was formed basing on rule ofclearing away heat to detoxify and strengthening spleen to removedampness,in order to clarify the functional mechanism of Chang Yu NingGranula on treating active ulcerative colitis.Methods:Making rats models which according to the syndrome ofaccumulation of dame-heat by using composite methods and then treatingthem with Chang Yu Ning Granula.Both of the two Chinese medicinetreating groups were given high dose and low dose of Chang Yu NingGranula separately.The control group was treated with SASP,meanwhile,there are also model group and blank group for comparison.The treatinggroup was administrated for 4 weeks after successfully making rats modelsfor 16 days.Inflammatory activity index(DAI)was assessed during modelsmaking period and after treatment.The tissues from rats models wereobserved under optical microscope,in order to know the change or gastricmucosa and the pathological and histological injury,then the expression ofTLR4,NF-kB and TNF-a were detected in tissues with Elisa methods andRT-PCR respectively.Result:Results:It is indicated by statistical analysis that the scores ofDAI,histological injury,and general morphological damage weresignificantly different in blank control group,control group,westernmedicine group,low dose of Chinese medicine group and high dose ofChinese medicine group.(p＜0.01),There weren't obvious difference amongwestern medicine group,low dose group of Chinese medicine and high dosegroup of Chinese medicine.It shows that both of Chang Yu Ning Granulaand SASP have therapeutic effect with UC.and Chang Yu Ning Granula has regulating function on cytokine network factors TLR4,NF-kB and TNF-a.Conclusion:1.The basic pathogenesis of active UC is mainlyaccumulation of damp-heat accompany with deficiency of spleen.Accumulation of damp-heat is the pathological characteristic of active UCand deficiency of spleen is basic pathogenesis and inevitable outcome.2.Cytokine network factors TLR4,NF-kB and TNF-a in rats colonic mucosahave high expression in active UC,which may be important mechanism ofincidence of UC.3.Chang Yu Ning Granula has therapeutic effects on activeUC,and can also low down the score of DAI,general morphology andinflammation.4.Chang Yu Ning Granula has inhibition function on theexpression of TLR4 in rats colonic mucosa of active UC.5.Chang Yu NingGranula has inhibition function on the expression of TNF-a in rats colonicmucosa of active UC.6.Chang Yu Ning Granula has inhibition function onthe expression of NF-kB in rats colonic mucosa of active UC.7.Chang YuNing Granula has regulating function on expression of cytokine networkfactors in rats colonic mucosa of active UC,which is the main mechanismof Chang Yu Ning Granula on treating UC.