The. Qingdan Diarrhea Mechanism Of Heat Treatment Of Bi Yuan

Objective:To study the mechanism of action about the method of clearing gallbladder and purging heat to Bi Yuan at the gene level on molecular biology and molecular biology by animal experiment,then to guide the clinical medication more accurately.Methods:Group the healthy SD rats into the following six parts randomly:blank group,model group,Ji Lei Kai Qiao Tang low-dose group,Ji Lei Kai Qiao Tang middle-dose,Ji Lei Kai Qiao Tang high-dose and penicillinum v potassium group. According to the method reported by YE Jing we made the Acute Rhinosinusitis model.In the following seven days we drenched the blank group and the model group 5ml·kg^-1·d^-1 with the sterile water(SW),and the other four groups with corresponding medicine.All these days we observed the changes of the rats about the pH value,body temperature,and body weight,especially the nose symptom and secretory volume. After seven days we killed these rats and chose six in each group randomly.We measured the C-reactive protein(CRP) concentration in the SD rats' blood serum using the ELISA method.First we took out of the mucous membrane of nose at the superclean bench,after that we extracted the mRNA and reversed transcription to cDNA,then we measured the expressions of IL-2 mRNA,IL-4 mRNA,IL-10 mRNA and IFN-γmRNA by using the RT-PCR method.At last we observed the morphologic changes of the mucous membrane of nose under the electron microscope.Results:1.Comparing with the blank group,the pH value of the model group's nasal cavity secretion substance has improved significantly(P＜0.01).The nasal cavity secretion substance's pH values of all the therapy groups have declined,especially the Ji Lei Kai Qiao Tang low-dose group.2.Comparing with the blank group,the blood serum CRP concentration of the model group has improved significantly(P＜0.01).The blood serum CRP concentrations of all the therapy groups have declined especially the Ji Lei Kai Qiao Tang low-dose group and the middle-dose group(P＜0.01).3.After seven days medication,except the Ji Lei Kai Qiao Tang high-dose group, the expressions of IL-2 mRNA and IL-10 mRNA of all the therapy groups have improved especially the Ji Lei Kai Qiao Tang low-dose group and the penicillinum v potassium group(P＜0.01).4.After seven days medication,except the Ji Lei Kai Qiao Tang high-dose group, the expressions of IL-4 mRNA of all the therapy groups have improved especially the Ji Lei Kai Qiao Tang low-dose group(P＜0.01).5.After seven days medication,the expressions of IFN-γmRNA of all the therapy groups have declined especially the Ji Lei Kai Qiao Tang low-close group and the middle-dose group(P＜0.01).6.After seven days medication,through the transmission electron microscope we find that except the Ji Lei Kai Qiao Tang high-dose group,the membrane of nose cilia of all the therapy groups have returned to normal gradually especially the Ji Lei Kai Qiao Tang low-dose group and the middle-dose group.Conclusion:1.By falling the Nasal discharge pH value to normal,clearing gallbladder and purging heat can make the internal environment concordance of the nasal cavity.2.Clearing gallbladder and purging heat can reduce the CRP concentration of the blood serum,depression or kill the causative organism,and eliminate the inflammatory reaction.3.Clearing gallbladder and purging heat can strengthen the expression of the nasal mucosa IL-2 mRNA,act on the T cell to strengthen the cell immunity and improve the anti-infection ability of the organism.4.Clearing gallbladder and purging heat can strengthen the expression of the nasal mucosa IL-4 mRNA and IL-10 mRNA,as a result to educe the anti-inflammatory activity.5.Clearing gallbladder and purging heat can restrain the expression of the nasal mucosa IFN-γmRNA,as a result to restrain the synthesization of the correlated inflammatory factor.6.Clearing gallbladder and purging heat can lighten the broaden of the endocytoplasmic reticulum and the engorgement of the mitochondria,as a result to promote the nasal Mucus cilia transmission system to return to normal.We suppose that Ji Lei Kai Qiao Tang can improve organism internal environment by the method of clearing gallbladder and purging heat,strengthening vital qi to eliminate pathogenic factor.