The Study Of RALP And RRR's Different Compatibility Levels On SYDC'S Effects And Active Mechanisms

Objective:The aims of this article are to reveal the compatibility rules of traditional Chinese medicine(TCM),and to offer the basis for scientific application of Chinese drugs in the clinic according to the research of Radix aconiti lateralis preparata(RALP) and Radix et Rhizoma Rhei(RRR) in treating the syndrome of yang-deficiency and constipation(SYDC).Methods:On the basis of establishing animal models with SYDC,through the experiments of excreting feces,observing alvine movement and colonic myoelectricity,determining intestine-pathology and correlative indexes to study effective mechanisms with the cut crude drug-compatibility,constituent-compatibility of RALP and RRR in treating SYDC.Selecting colon's interstitial cells of Cajal(CICC) of KM's suckling mouse to study active mechanisms of RALP and RRR' component-compatibility on CICC.Results:The results indicated that the compatibility of RALP and RRR at different ratios in treating SYDC were better than single drug of both,whose effect mechanism might be related to the content changes of gastrointestinal hormones and nervous transmitters;At the prophylactic administration drug,the compatibility of both total alkaloid of RALP and RRR's total anthraquinone in ratio of 2:1 and the total alkaloid of RALP and RRR's conjunct anthraquinone in ratios of 2:1 and 1:1 had effect on synergizing action;However,at the therapeutic administration drug,the compatibility of the total alkaloid of RALP and RRR's total anthraquinone in ratio of 1:2 was optimal in synergizing effect.The effective mechanisms of the compatibility among components in curing SYDC might be related to the content changes of MTL,SS and AchE in blood plasma.Aconitine and emodin are toxic to CICC, maximal toxicity dose and critical time of both were 0.1%,1min,15min,30min, 60min,120min,180min,240min;0.5%,1min,15min,30min.The compatibility ratio(2:1) of aconitine and emodin had effectiveness in decreasing aconitine's toxicity.Probably,the active mechanisms were inhibiting cell membrane's damnification,the debaser of the livingness of Na⁺K⁺-ATPase and the content of Ca²⁺ that lead by aconitine.Conclusions:This study shows that the optimal ratio of the compatibility of the total alkaloid of RALP and RRR's total and conjunct anthraquinone in treating SYDC at the prophylactic administration drug and therapeutic administration drug are respectively 2:1 and 1:2.This study also shows that the compatibility of aconitine and emodin(2:1)has effect on CICC's decreasing toxicity and improving action.