The Influences Brought By Gubijian Formula In The Process Of Establishing Experimental Rabbit Models Of Osteoarthritis Induced By The Joint Immobilization Method

Objective:Through the comparison study on the therapy influences, which are brought by Gubijian Formula, in the process of establishing experimental rabbit model of osteoarthritis introduced by the joint immobilization method, its preventive effect of early treatment against osteoarthritis and its safety of medical treatment are evaluated. Through the observations made to changes of joint functions and joint cartilages,and adjustment functions to matrix metalloproteinases-1 , which are corresponding to pre-treated,synch-treated and deferred treatment experimental rabbit groups,during the process of establishing rabbit models of osteoarthritis induced by the joint immobilization method, the evaluation to the best period of the early preventive treatment against osteoarthritis using Gubijian Formula is performed. In the field of osteoarthritis treatment, the thesis provides the experimental basis to the theories of Chinese traditional medicine, which are"To take the preventive treatment is a doctor's first priority"and"The doctor should pay great attention to people who are in their sub-health conditions". In this article, the discussion regarding the preventive strategy against osteoarthritis,which originates from the chinese traditional medicine,is also developed.Designs: 1. Forty healthy New Zealand white rabbits are selected and divided to four groups randomly. As soon as the process of establishing experimental rabbit model of osteoarthritis is started,one group is given the treatment of Gubijian Formula and another group is given the treatment of glucosamine sulfate. These two groups are the medicine -treated control groups. Also, the blank control group and the control group that is only for establishing the model of osteoarthritis are organized. The process of establishing the experimental rabbit model of osteoarthritis is started by adopting the joint immobilization method. After 30 days , the observation is made to the clinical performances of joints that are used to establish the model of osteoarthritis,main situations of joint cartilages and pathological changes under the light microscope,and the monitoring is performed to the blood routine examinations,hepatic and renal functions and weights of each control group. 2. Thirty healthy New Zealand white rabbits are selected and divided to three groups randomly. The synch-treated control group is treated with Gubijian Formula as soon as the process of establishing the model of osteoarthritis is started. The pre-treated control group was treated with Gubijian Formula a few days before the process is started. The last group with the deferred treatment will be treated with Gubijian Formula a few days after the process is started. The process of establishing the experimental rabbit model of osteoarthritis is started by adopting the joint immobilization method. After 30 days, the observation is made to the clinical performances of joints that are used to establish the model of osteoarthritis,main situations of joint cartilages and pathological changes under the light microscope,and the monitoring is performed to the blood routine examinations,hepatic and renal functions and weights of each control group. At the same time,with the immunohistochemical method, the observation is made to the expression of matrix metalloproteinase-1 in cartilages.Results: 1. The grade of clinical performance gotten from the control group treated with Gubijian Formula is much better than the control group that is only for establishing the experimental model of osteoarthritis(P<0.05),and does not have significant difference with the group treated with the glucosamine sulfate(P>0.05). The evaluation of main situations of cartilages and pathological changes under the light microscope,which is gotten from the control group with the treatment of Gubijian Formula, is much better than the control group with the pure model establishment of osteoarthritis,and does not have the significant difference with the control group treated by the glucosamine sulfate. Mankin's Marks that are gotten from the control group with the treatment o f the Gubijian Formula and the control group with the pure model establishment of osteoarthritis have a significant difference(P<0.01),but Mankin's Marks of two groups with the Gubijian Formula treatment and the glucosamine sulfate treatment do not have a significant difference(P>0.05). There is no significant difference in the safety indexes of four control groups (P>0.05). Comparing to the other three control groups, there are some significant differences in the blank control group in terms of the clinical performance , situations of cartilages and pathological changes under the light microscope and the Mankin's Mark(P<0.05). 2. The indexes of clinical performance including the indexes of local pain reaction and joint swelling, which is gotten from the pre-treated control group, are much better than the synch-treated and deferred treatment control groups(P<0.05). The indexes of the gait change and motion range of joints, which are gotten from the pre-treated control group, are much better than the deferred treatment group and do not have the significant difference with the synch-treated group(P>0.05).Every index of clinical performance gotten from synch-treated group is much better than the deferred treatment group(p<0.05). The main situations of cartilages,evaluation of pathological changes under the light microscope and Mankin's Mark,which are gotten from the pre-treated control group,are much better than the synch-treated control group. The main situations of cartilages,evaluation of pathological changes under the light microscope and Mankin's Mark,which are gotten from the synch-treated control group,are much better than the deferred treatment control group(P<0.05).The expression rate of Matrix Metalloproteinase-1 of the pre-treated control group is much lower than the synch-treated control group(P<0.05). The expression rate of Matrix Metalloproteinase-1 of the synch-treated control group is much lower than the deferred treatment control group(P<0.05).There is no significant difference in the safety indexes of three control groups (P>0.05).Conclusions: 1. The Gubijian Formula can effectively reduce the pathological injury intensity caused by the process of establishing the experimental rabbit model of osteoarthritis introduced by the joint immobilization method,and improve the clinical performance and histological grade. The control group that was treated with The Gubijian Formula before the model establishment of osteoarthritis has the most obvious medical effect. 2. The Gubijian Formula can control the activation of Matrix Metalloproteinase-1 of rabbits'joint cartilages and adjust the cytokines. Therefore,it can inhibit the egradation of the cartilage matrixes during the process of establishing the model of osteoarthritis. The control group that was treated with The Gubijian Formula before the model establishment of osteoarthritis has the most obvious adjustment function. 3. Although the formula can not prevent the pathological development of the experimental osteoarthritis,it can slow down its progress. The control group that is treated early has better therapeutic effect than the control group that is treated late. No obvious adverse influence is found in the blood indexes and hepatic and renal functions of experimental rabbits.