The Interactional Effects Of Micronutrients And Phytochemical On The Oxidative Stress And Cognitive

Background:Chronic Non-Communicable Disease (NCD) plays an importantrols in human health issues in the past dedecades.Free radicals andoxidative stress have been implicated to the pathogenesis of NCD.But therewas few direct evidence indicating that individual antioxidant showssignificantly effect on the prevention of NCD.The potential synergism ofantioxidants may help to achieve the goal of NCD prevention.Objectives:To study the relationship of phytochemicals and antioxidativenutrients in against oxidant stress in vivo.To explore the potentialmechanism of combined phytochemicals and antioxidative nutrientssupplements on antioxidant and NCD prevention.Methods:High-fat intake animal model was used to study the protection ofcarotenoids (Caro) and ginkgo flavonoids (GF),carotenoids and zinc +selenium (ZS) against the oxidative stress.The the memory improving effectof carotenoids and zinc + selecium supplements in mice was also evaluated.Results:1.Carotenoids supplyment either individually or combined reduced theendogeneis DNA damage of high-fat intake rats significantly.Significantdecreases of endogenesis DNA damage were observed whileas the high-fatintake rats administrated GF,GF+Caro or ZS+Caro.A synergy trend ofcarotenoids and zinc+selenium on DNA damage protection was observed inthe study.2.Carotenoids,ZS or ginkgo flavonoids supplyment either individually or combined increased the membrain lipid fluidity of neural cells.No evidencewas observed that indicated the synergy of the antioxidants.3.Lycopene andβ-carotene supplyment either individually or combinedincreased the plasma HDL-c level and modificate the content of plasma lipids.Lycopene andβ-carotene may work together on blood lipid reducing andprotecting blood lipid against peroxidation.Combined Caro and GF trend tocooperate in lipid reducing.4.Individually supplyment of lutein,lycopene andβ-carotene decreased thelipid peroxidation level in hight-fat intake rats,but there was no significantinteractive evidence for the carotenoids on against lipid perxidation.GFsupplyment can lower the MDA levels of the rats' tissues.Caro and GF aretrend to synergy in decrease the lipid peroxidation in mice but not in rats.Combination of Caro and ZS could increase the SOD and GSH-Px levels ofhigh-fat intake rats' neural cells.5.Mixed Caro and mixed Caro + GF supplyment improved the positiverememberance and active learning abilities of mice and increase the memorymaintaining.Carotenoids and GF are trend to synergy on enhancing mice'smemory ability.Conclusions:Carotenoids,ginkgo flavonoids and zinc + selenium worked indifferent ways to reduce the risk of oxidative stress and enhance the ability ofantioxidant in rats and mice in vivo.Combined carotenoids and ginkgoflavonoids or zinc + selenium supplyments showed their prospectivepotentiality on reducing the free radical and oxidant damages and desreaseingrisks of NCD.Further sutudies need to be done on the synergy of theantioxidants.